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- W2912462188 abstract "Cellular adhesion is dependent on the type of adhesion receptors displayed on the cell surface, and activation of adhesion receptors initiates downstream signaling cascades that are context dependent. Cellular expression of adhesion receptors is modulated by a complex array of extracellular and intracellular signals, and we have found that Inositol Phosphate Multikinase (IPMK) is a transcriptional regulator of integrin β1 protein in murine embryonic fibroblast (MEF) cells. Further, we show that although loss of IPMK dramatically reduces levels of both active and inactive integrin β1 protein, activation of the focal adhesion proteins FAK and Src are increased while total protein levels are unchanged, suggesting that IPMK is a negative regulator of focal adhesion complex activity. Importantly, IPMK regulation of integrin gene expression is activity dependent. Using native extracellular matrix mimicking fibers as substrate, generated using STEP (Spinneret-based Tunable Engineered Parameters) technique, we show that loss of this enzyme inhibits migration (0.68 μm/min in Wild Type cells vs 0.35 μm/min in IPMK Knock-out cells) due to decreased cell-matrix adhesion and reduced cellular contractility. Using nanonet force microscopy (NFM), we calculated the forces of migrating MEF WT cells to be 226.8 nN which was significantly higher than those of the IPMK KO cells (115.6 nN). Attempts to rescue IPMK pharmacologically resulted in increased contractility (154.3 nN) but not to the same degree as that of WT cells. To further elucidate the impact of IPMK on adhesion strength, we stretched the cells suspended between fibers until their adhesion bonds failed and the cells detached from the adhered fibers. WT cells demonstrated higher failure force (45.4 nN) as opposed to KO cells (36.2 nN). Based on these findings, we hypothesize that IPMK modulates cell migration and contractility by regulating gene expression of cell-matrix molecules." @default.
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- W2912462188 date "2019-02-01" @default.
- W2912462188 modified "2023-09-30" @default.
- W2912462188 title "IPMK Loss Inhibits Cellular Motility and Contractility" @default.
- W2912462188 doi "https://doi.org/10.1016/j.bpj.2018.11.2941" @default.
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