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- W2912488502 abstract "背景IncFII-FIA-FIB型质粒广泛存在于肠杆菌科细菌中，介导了许多耐药基因的水平转移，并导致细菌多重耐药问题日益严重。 目的分析IncFII-FIA-FIB型多重耐药质粒pBTR-CTXM的基因组结构，并研究其介导大肠杆菌BTR株的耐药基因水平转移机制。 方法利用PCR进行耐药基因筛查；接合转移和电转化实验验证质粒pBTR-CTXM是否具备自主接合转移的特性；VITEK 2 Compact全自动细菌鉴定及药敏分析仪测定相关菌株对抗生素的药物敏感性；构建Mate Pair文库并进行细菌全基因组高通量测序和质粒结构基因组学分析。 结果菌株BTR是携带blaNDM-1、blaCTX-M-15、blaTEM、qnrD、qnrS1、mph(A)、erm(B)和tetA(B)等耐药基因的多重耐药大肠杆菌，其中blaCTX-M-15、mph(A)、erm(B)和tetA(B)等耐药基因均位于大小为144 939 bp的质粒pBTR-CTXM (GenBank登录号MF156697)上，该质粒可与菌株BTR内质粒pNDM-BTR接合共转移到受体菌大肠杆菌EC600中。pBTR-CTXM具备IncFII-FIA-FIB型质粒典型的骨架区结构，其多重耐药(Multidrug-resistant，MDR)区由新的复合型转座子Tn6492、Tn2残余、Tn10残余、ISEcp1-blaCTX-M-15-Δorf477转座单元和一些插入序列组成。 结论pBTR-CTXM中新复合型转座子Tn6492与Tn10残余和ISEcp1-blaCTX-M-15-Δorf477转座单元共同介导大肠杆菌BTR株的多重耐药与耐药基因的水平传播。" @default.
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- W2912488502 date "2019-01-20" @default.
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- W2912488502 title "IncFII-FIA-FIB型多重耐药质粒pBTR-CTXM的结构基因组学分析" @default.
- W2912488502 doi "https://doi.org/10.13344/j.microbiol.china.180431" @default.
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