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- W2912497511 abstract "Real‐time assessment of excised tissue may help to improve surgical results in breast tumor surgeries. Here, as a step towards this purpose, the potential of second and third harmonic generation (SHG, THG) microscopy is explored. SHG and THG are nonlinear optical microscopic techniques that do not require labeling of tissue to generate 3D images with intrinsic depth‐sectioning at sub‐cellular resolution. Until now, this technique had been applied on fixated breast tissue or to visualize the stroma only, whereas most tumors start in the lobules and ducts. Here, SHG/THG images of freshly excised unprocessed healthy human tissue are shown to reveal key breast components—lobules, ducts, fat tissue, connective tissue and blood vessels, in good agreement with hematoxylin and eosin histology. DNA staining of fresh unprocessed mouse breast tissue was performed to aid in the identification of cell nuclei in label‐free THG images. Furthermore, 2‐ and 3‐photon excited auto‐fluorescence images of mouse and human tissue are collected for comparison. The SHG/THG imaging modalities generate high quality images of freshly excised tissue in less than a minute with an information content comparable to that of the gold standard, histopathology. Therefore, SHG/THG microscopy is a promising tool for real‐time assessment of excised tissue during surgery." @default.
- W2912497511 created "2019-02-21" @default.
- W2912497511 creator A5003751896 @default.
- W2912497511 creator A5011980870 @default.
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- W2912497511 date "2019-03-21" @default.
- W2912497511 modified "2023-10-10" @default.
- W2912497511 title "Second and third harmonic generation microscopy visualizes key structural components in fresh unprocessed healthy human breast tissue" @default.
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- W2912497511 doi "https://doi.org/10.1002/jbio.201800297" @default.
- W2912497511 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7065644" @default.
- W2912497511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30684312" @default.
- W2912497511 hasPublicationYear "2019" @default.
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