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- W2912504243 abstract "The development of fluorescence-based oxygen sensors coupled with microplate-based assays for quantitative bioenergetics analyses enables screening multiple experimental conditions at once with small biological material and in a timely manner. In this chapter, we outline detailed protocols and practical tips to design and perform controlled measurements of (a) respiratory and glycolytic metabolism of intact cells, (b) substrate-dependent respiration in permeabilized cells and isolated mitochondria, and (c) calcium-dependent regulation of mitochondrial bioenergetics with Seahorse XF Flux Analyzers." @default.
- W2912504243 created "2019-02-21" @default.
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- W2912504243 date "2019-01-01" @default.
- W2912504243 modified "2023-10-13" @default.
- W2912504243 title "Assessing Calcium-Stimulated Mitochondrial Bioenergetics Using the Seahorse XF96 Analyzer" @default.
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- W2912504243 doi "https://doi.org/10.1007/978-1-4939-9018-4_18" @default.
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