FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912510006> ?p ?o ?g. }

• W2912510006 endingPage "487" @default.
• W2912510006 startingPage "475" @default.
• W2912510006 abstract "Bacitracin is a cell wall targeting antimicrobial with clinical and agricultural applications. With the growing mismatch between antimicrobial resistance and development, it is essential we understand the molecular mechanisms of resistance in order to prioritize and generate new effective antimicrobials. BcrR is a unique membrane-bound one-component system that regulates high-level bacitracin resistance in Enterococcus faecalis . In the presence of bacitracin, BcrR activates transcription of the bcrABD operon conferring resistance through a putative ATP-binding cassette (ABC) transporter (BcrAB). BcrR has three putative functional domains, an N-terminal helix–turn–helix DNA-binding domain, an intermediate oligomerization domain and a C-terminal transmembrane domain. However, the molecular mechanisms of signal transduction remain unknown. Random mutagenesis of bcrR was performed to generate loss- and gain-of-function mutants using transcriptional reporters fused to the target promoter P bcrA . Fifteen unique mutants were isolated across all three proposed functional domains, comprising 14 loss-of-function and one gain-of-function mutant. The gain-of-function variant (G64D) mapped to the putative dimerization domain of BcrR, and functional analyses indicated that the G64D mutant constitutively expresses the P bcrA-luxABCDE reporter. DNA-binding and membrane insertion were not affected in the five mutants chosen for further characterization. Homology modelling revealed putative roles for two key residues (R11 and S33) in BcrR activation. Here we present a new model of BcrR activation and signal transduction, providing valuable insight into the functional characterization of membrane-bound one-component systems and how they can coordinate critical bacterial responses, such as antimicrobial resistance." @default.
• W2912510006 created "2019-02-21" @default.
• W2912510006 creator A5020052351 @default.
• W2912510006 creator A5025438688 @default.
• W2912510006 creator A5042268753 @default.
• W2912510006 creator A5048049913 @default.
• W2912510006 creator A5060080435 @default.
• W2912510006 date "2019-04-01" @default.
• W2912510006 modified "2023-10-03" @default.
• W2912510006 title "Functional characterization of BcrR: a one-component transmembrane signal transduction system for bacitracin resistance" @default.
• W2912510006 cites W1497492892 @default.
• W2912510006 cites W1505235074 @default.
• W2912510006 cites W1613849333 @default.
• W2912510006 cites W1965570469 @default.
• W2912510006 cites W1965614061 @default.
• W2912510006 cites W2009649338 @default.
• W2912510006 cites W2010700017 @default.
• W2912510006 cites W2026365809 @default.
• W2912510006 cites W2027631648 @default.
• W2912510006 cites W2030742563 @default.
• W2912510006 cites W2034541624 @default.
• W2912510006 cites W2039123232 @default.
• W2912510006 cites W2044149388 @default.
• W2912510006 cites W2055351105 @default.
• W2912510006 cites W2060913542 @default.
• W2912510006 cites W2072108430 @default.
• W2912510006 cites W2078989762 @default.
• W2912510006 cites W2102964870 @default.
• W2912510006 cites W2107093868 @default.
• W2912510006 cites W2108047199 @default.
• W2912510006 cites W2113518661 @default.
• W2912510006 cites W2117491496 @default.
• W2912510006 cites W2124102845 @default.
• W2912510006 cites W2145866505 @default.
• W2912510006 cites W2147792727 @default.
• W2912510006 cites W4211156111 @default.
• W2912510006 cites W2091841072 @default.
• W2912510006 doi "https://doi.org/10.1099/mic.0.000781" @default.
• W2912510006 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30777814" @default.
• W2912510006 hasPublicationYear "2019" @default.
• W2912510006 type Work @default.
• W2912510006 sameAs 2912510006 @default.
• W2912510006 citedByCount "6" @default.
• W2912510006 countsByYear W29125100062020 @default.
• W2912510006 countsByYear W29125100062022 @default.
• W2912510006 countsByYear W29125100062023 @default.
• W2912510006 crossrefType "journal-article" @default.
• W2912510006 hasAuthorship W2912510006A5020052351 @default.
• W2912510006 hasAuthorship W2912510006A5025438688 @default.
• W2912510006 hasAuthorship W2912510006A5042268753 @default.
• W2912510006 hasAuthorship W2912510006A5048049913 @default.
• W2912510006 hasAuthorship W2912510006A5060080435 @default.
• W2912510006 hasBestOaLocation W29125100061 @default.
• W2912510006 hasConcept C104317684 @default.
• W2912510006 hasConcept C118892022 @default.
• W2912510006 hasConcept C143065580 @default.
• W2912510006 hasConcept C15152581 @default.
• W2912510006 hasConcept C16318435 @default.
• W2912510006 hasConcept C170493617 @default.
• W2912510006 hasConcept C24530287 @default.
• W2912510006 hasConcept C2779860364 @default.
• W2912510006 hasConcept C501593827 @default.
• W2912510006 hasConcept C54355233 @default.
• W2912510006 hasConcept C55493867 @default.
• W2912510006 hasConcept C62478195 @default.
• W2912510006 hasConcept C86803240 @default.
• W2912510006 hasConcept C95444343 @default.
• W2912510006 hasConceptScore W2912510006C104317684 @default.
• W2912510006 hasConceptScore W2912510006C118892022 @default.
• W2912510006 hasConceptScore W2912510006C143065580 @default.
• W2912510006 hasConceptScore W2912510006C15152581 @default.
• W2912510006 hasConceptScore W2912510006C16318435 @default.
• W2912510006 hasConceptScore W2912510006C170493617 @default.
• W2912510006 hasConceptScore W2912510006C24530287 @default.
• W2912510006 hasConceptScore W2912510006C2779860364 @default.
• W2912510006 hasConceptScore W2912510006C501593827 @default.
• W2912510006 hasConceptScore W2912510006C54355233 @default.
• W2912510006 hasConceptScore W2912510006C55493867 @default.
• W2912510006 hasConceptScore W2912510006C62478195 @default.
• W2912510006 hasConceptScore W2912510006C86803240 @default.
• W2912510006 hasConceptScore W2912510006C95444343 @default.
• W2912510006 hasIssue "4" @default.
• W2912510006 hasLocation W29125100061 @default.
• W2912510006 hasLocation W29125100062 @default.
• W2912510006 hasLocation W29125100063 @default.
• W2912510006 hasOpenAccess W2912510006 @default.
• W2912510006 hasPrimaryLocation W29125100061 @default.
• W2912510006 hasRelatedWork W188926022 @default.
• W2912510006 hasRelatedWork W1976616850 @default.
• W2912510006 hasRelatedWork W2009647017 @default.
• W2912510006 hasRelatedWork W2028870752 @default.
• W2912510006 hasRelatedWork W2148699601 @default.
• W2912510006 hasRelatedWork W2357213098 @default.
• W2912510006 hasRelatedWork W2358692005 @default.
• W2912510006 hasRelatedWork W2391638901 @default.
• W2912510006 hasRelatedWork W2505736768 @default.
• W2912510006 hasRelatedWork W58491693 @default.
• W2912510006 hasVolume "165" @default.

• next