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• W2912555315 abstract "In this study, we measured the effect of EPS on Cd and proton adsorption behaviors by measuring the extent of adsorption onto biomass with and without the EPS removed via a cation exchange resin. We conducted both Cd adsorption experiments and potentiometric titrations of biomass using three common bacterial species: one Gram-positive (Bacillus subtilis) and two Gram-negative (Shewanella oneidensis, Pseudomonas putida) species. The Cd adsorption experiments were conducted as a function of metal loading in order to probe whether environmentally-low metal loadings lead to different adsorption mechanisms and roles for EPS than the higher metal loadings of most previous adsorption studies. We suspended each biomass sample in a solution of dissolved Cd in 0.01 M NaClO4 at metal loadings of 1, 2, 5, and 74 μmol/g. Surface complexation modeling (SCM) was used to determine stability constants for the important Cd-bacteria complexes, and the effect of metal loading on the resulting calculated stability constant values was determined.In general, the measured bulk Cd adsorption behavior is unaffected by EPS removal. However, our potentiometric titration results suggest that EPS removal does alter the distribution of site types, but not the mass-normalized total site concentration within the biomass. SCM suggests that high affinity sulfhydryl sites control Cd binding under low metal loading conditions for B. subtilis and P. putida, and that sulfhydryl sites are present both on the cells and within the EPS for these species. Conversely, the SCM results suggest that Cd-sulfhydryl binding is un-important on the EPS of S. oneidensis." @default.
• W2912555315 created "2019-02-21" @default.
• W2912555315 creator A5070557993 @default.
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• W2912555315 date "2019-01-28" @default.
• W2912555315 modified "2023-10-14" @default.
• W2912555315 title "Influence of Extracellular Polymeric Substances on the Adsorption of Cadmium onto Three Bacterial Species" @default.
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• W2912555315 doi "https://doi.org/10.1080/01490451.2018.1564804" @default.
• W2912555315 hasPublicationYear "2019" @default.
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