FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912559104> ?p ?o ?g. }

• W2912559104 endingPage "101017" @default.
• W2912559104 startingPage "101017" @default.
• W2912559104 abstract "Palm oil mill effluent (POME) is a major waste from oil-palm industries often disposed indiscriminately into surrounding water bodies, adding up to retarding soil health and environmental pollution. Here, POME was beneficially used for lipase production by newly isolated microorganisms from oil-polluted soil. The identified microorganisms, using 16S rRNA gene analysis, were screened for lipolytic activity and treated with ethidium bromide for possible mutation in nucleotide sequence and enhancement of their lipolytic potential. A mutant strain of Bacillus niacini as confirmed by CodonCode aligner software showed the highest lipolytic activity compared to the wild type organisms. The crude lipase produced from the mutant strain was purified to apparent homogeneity using ammonium sulphate precipitation, ion-exchange chromatography on DEAE-Sephadex A-50 and gel-filtration on Sephadex G-100. Some biochemical properties of the lipase were studied prior to its application for the hydrolysis of different oils. The 59 kDa purified lipase exhibited optimal activity at pH 9.0 and 40 °C. The enzyme is alkaline thermostable being active at pH 8.0–12 and retained about 60% of initial activity after 60 min of incubation at 90 °C. At 10 mM salt concentration, Al3+ and Ca2+ modulated the activity of the enzyme but inhibited by Cu2+, Hg2+, EDTA, β-mercaptoethanol, SDS and Urea. The Km and Vmax of the lipase with olive oil as substrate are respectively, 6.12 mg/mL and 59.64 μmol/min/mg as the enzyme was able to hydrolyze various natural oils. These remarkable properties make the purified EMB-5 lipase employable for biotechnological applications in lipid-utilizing industries." @default.
• W2912559104 created "2019-02-21" @default.
• W2912559104 creator A5030054858 @default.
• W2912559104 creator A5055846671 @default.
• W2912559104 creator A5058824768 @default.
• W2912559104 creator A5060680927 @default.
• W2912559104 creator A5071069732 @default.
• W2912559104 date "2019-03-01" @default.
• W2912559104 modified "2023-10-12" @default.
• W2912559104 title "Enhanced lipolytic activity potential of mutant Bacillus niacini EMB-5 Grown on Palm Oil Mill Effluent (POME) and biochemical characterization of purified lipase" @default.
• W2912559104 cites W1843633333 @default.
• W2912559104 cites W1979292909 @default.
• W2912559104 cites W1987075026 @default.
• W2912559104 cites W1988734325 @default.
• W2912559104 cites W1991867703 @default.
• W2912559104 cites W1996644470 @default.
• W2912559104 cites W1998656473 @default.
• W2912559104 cites W2008020809 @default.
• W2912559104 cites W2013268794 @default.
• W2912559104 cites W202530547 @default.
• W2912559104 cites W2029559315 @default.
• W2912559104 cites W2030646656 @default.
• W2912559104 cites W2049177736 @default.
• W2912559104 cites W2051872823 @default.
• W2912559104 cites W2055043387 @default.
• W2912559104 cites W2055920875 @default.
• W2912559104 cites W2059544403 @default.
• W2912559104 cites W2083598389 @default.
• W2912559104 cites W2100837269 @default.
• W2912559104 cites W2140026742 @default.
• W2912559104 cites W2154401636 @default.
• W2912559104 cites W2158886877 @default.
• W2912559104 cites W2171212541 @default.
• W2912559104 cites W2293720079 @default.
• W2912559104 cites W2311203695 @default.
• W2912559104 cites W2316038605 @default.
• W2912559104 cites W2328721385 @default.
• W2912559104 cites W2337437675 @default.
• W2912559104 cites W2530173156 @default.
• W2912559104 cites W2552840967 @default.
• W2912559104 cites W2579353171 @default.
• W2912559104 cites W4249967326 @default.
• W2912559104 cites W4293247451 @default.
• W2912559104 cites W801829339 @default.
• W2912559104 doi "https://doi.org/10.1016/j.bcab.2019.01.055" @default.
• W2912559104 hasPublicationYear "2019" @default.
• W2912559104 type Work @default.
• W2912559104 sameAs 2912559104 @default.
• W2912559104 citedByCount "4" @default.
• W2912559104 countsByYear W29125591042021 @default.
• W2912559104 countsByYear W29125591042022 @default.
• W2912559104 countsByYear W29125591042023 @default.
• W2912559104 crossrefType "journal-article" @default.
• W2912559104 hasAuthorship W2912559104A5030054858 @default.
• W2912559104 hasAuthorship W2912559104A5055846671 @default.
• W2912559104 hasAuthorship W2912559104A5058824768 @default.
• W2912559104 hasAuthorship W2912559104A5060680927 @default.
• W2912559104 hasAuthorship W2912559104A5071069732 @default.
• W2912559104 hasConcept C143108565 @default.
• W2912559104 hasConcept C163994747 @default.
• W2912559104 hasConcept C175605896 @default.
• W2912559104 hasConcept C181199279 @default.
• W2912559104 hasConcept C185592680 @default.
• W2912559104 hasConcept C2779697368 @default.
• W2912559104 hasConcept C2780786110 @default.
• W2912559104 hasConcept C28212737 @default.
• W2912559104 hasConcept C31903555 @default.
• W2912559104 hasConcept C43617362 @default.
• W2912559104 hasConcept C523546767 @default.
• W2912559104 hasConcept C54355233 @default.
• W2912559104 hasConcept C55493867 @default.
• W2912559104 hasConcept C59822182 @default.
• W2912559104 hasConcept C86803240 @default.
• W2912559104 hasConcept C94412978 @default.
• W2912559104 hasConceptScore W2912559104C143108565 @default.
• W2912559104 hasConceptScore W2912559104C163994747 @default.
• W2912559104 hasConceptScore W2912559104C175605896 @default.
• W2912559104 hasConceptScore W2912559104C181199279 @default.
• W2912559104 hasConceptScore W2912559104C185592680 @default.
• W2912559104 hasConceptScore W2912559104C2779697368 @default.
• W2912559104 hasConceptScore W2912559104C2780786110 @default.
• W2912559104 hasConceptScore W2912559104C28212737 @default.
• W2912559104 hasConceptScore W2912559104C31903555 @default.
• W2912559104 hasConceptScore W2912559104C43617362 @default.
• W2912559104 hasConceptScore W2912559104C523546767 @default.
• W2912559104 hasConceptScore W2912559104C54355233 @default.
• W2912559104 hasConceptScore W2912559104C55493867 @default.
• W2912559104 hasConceptScore W2912559104C59822182 @default.
• W2912559104 hasConceptScore W2912559104C86803240 @default.
• W2912559104 hasConceptScore W2912559104C94412978 @default.
• W2912559104 hasLocation W29125591041 @default.
• W2912559104 hasOpenAccess W2912559104 @default.
• W2912559104 hasPrimaryLocation W29125591041 @default.
• W2912559104 hasRelatedWork W183459390 @default.
• W2912559104 hasRelatedWork W2010457722 @default.
• W2912559104 hasRelatedWork W2013084152 @default.
• W2912559104 hasRelatedWork W2037347043 @default.
• W2912559104 hasRelatedWork W2081776302 @default.

• next