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- W2912581775 abstract "DNA cloning remains the primary step before the further investigation of gene function. Restriction enzyme-based cloning methods are still widely used and numerous restriction-free cloning techniques are available as alternatives. Here we describe a PCR-based cloning method named ABC cloning. This method uses PCR to combine three overlapping DNA fragments into a recombinant vector that can be immediately transformed into competent cells. This technique uses only a thermostable DNA polymerase and is more rapid and efficient than previously described methods." @default.
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- W2912581775 date "2019-01-01" @default.
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- W2912581775 title "ABC cloning: An efficient, simple, and rapid restriction/ligase-free method" @default.
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- W2912581775 doi "https://doi.org/10.1016/j.mex.2019.02.007" @default.
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