FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912603959> ?p ?o ?g. }

• W2912603959 endingPage "1720" @default.
• W2912603959 startingPage "1710" @default.
• W2912603959 abstract "Abstract The methanol‐regulated alcohol oxidase promoter (P AOX1 ) of Pichia pastoris (syn. Komagataella spp . ) is one of the strongest promoters for heterologous gene expression. Although increasing the gene dosage is a common strategy to improve recombinant protein productivities, P. pastoris strains harboring more than two copies of a Rhizopus oryzae lipase gene ( ROL ) have previously shown a decrease in cell growth, lipase production, and substrate consumption, as well as a significant transcriptional downregulation of methanol metabolism. This pointed to a potential titration effect of key transcriptional factors methanol expression regulator 1 (Mxr1) and methanol‐induced transcription factor (Mit1) regulating methanol metabolism caused by the insertion of multiple expression vectors. To prove this hypothesis, a set of strains carrying one and four copies of ROL (1C and 4C, respectively) were engineered to coexpress one or two copies of MXR1 *, coding for an Mxr1 variant insensitive to repression by 14‐3‐3 regulatory proteins, or one copy of MIT1. Small‐scale cultures revealed that growth, Rol productivity, and methanol consumption were improved in the 4C‐MXR1* and 4C‐MIT1, strains growing on methanol as a sole carbon source, whereas only a slight increase in productivity was observed for re‐engineered 1C strains. We further verified the improved performance of these strains in glycerol‐/methanol‐limited chemostat cultures." @default.
• W2912603959 created "2019-02-21" @default.
• W2912603959 creator A5028013763 @default.
• W2912603959 creator A5037504477 @default.
• W2912603959 creator A5051600714 @default.
• W2912603959 creator A5085984026 @default.
• W2912603959 date "2019-02-17" @default.
• W2912603959 modified "2023-10-16" @default.
• W2912603959 title "Deregulation of methanol metabolism reverts transcriptional limitations of recombinant <i>Pichia pastoris</i> ( <i>Komagataella</i> spp) with multiple expression cassettes under control of the <i>AOX1</i> promoter" @default.
• W2912603959 cites W1550242218 @default.
• W2912603959 cites W1572162748 @default.
• W2912603959 cites W1955127072 @default.
• W2912603959 cites W1985597601 @default.
• W2912603959 cites W1996245802 @default.
• W2912603959 cites W1999501626 @default.
• W2912603959 cites W2018200175 @default.
• W2912603959 cites W2020369705 @default.
• W2912603959 cites W2022775581 @default.
• W2912603959 cites W2029780957 @default.
• W2912603959 cites W2032013069 @default.
• W2912603959 cites W2046437093 @default.
• W2912603959 cites W2055775627 @default.
• W2912603959 cites W2055872607 @default.
• W2912603959 cites W2063070929 @default.
• W2912603959 cites W2068109582 @default.
• W2912603959 cites W2069940872 @default.
• W2912603959 cites W2075453882 @default.
• W2912603959 cites W2075946124 @default.
• W2912603959 cites W2081051355 @default.
• W2912603959 cites W2096971205 @default.
• W2912603959 cites W2098693002 @default.
• W2912603959 cites W2101678382 @default.
• W2912603959 cites W2105165430 @default.
• W2912603959 cites W2121264002 @default.
• W2912603959 cites W2122772601 @default.
• W2912603959 cites W2124479692 @default.
• W2912603959 cites W2144738246 @default.
• W2912603959 cites W2155333588 @default.
• W2912603959 cites W2158852468 @default.
• W2912603959 cites W2166894068 @default.
• W2912603959 cites W2169451027 @default.
• W2912603959 cites W2274846837 @default.
• W2912603959 cites W2279580433 @default.
• W2912603959 cites W2301304613 @default.
• W2912603959 cites W25146038 @default.
• W2912603959 cites W2695562666 @default.
• W2912603959 cites W2749487661 @default.
• W2912603959 cites W2767263894 @default.
• W2912603959 cites W2777118615 @default.
• W2912603959 doi "https://doi.org/10.1002/bit.26947" @default.
• W2912603959 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30712270" @default.
• W2912603959 hasPublicationYear "2019" @default.
• W2912603959 type Work @default.
• W2912603959 sameAs 2912603959 @default.
• W2912603959 citedByCount "17" @default.
• W2912603959 countsByYear W29126039592019 @default.
• W2912603959 countsByYear W29126039592020 @default.
• W2912603959 countsByYear W29126039592021 @default.
• W2912603959 countsByYear W29126039592022 @default.
• W2912603959 crossrefType "journal-article" @default.
• W2912603959 hasAuthorship W2912603959A5028013763 @default.
• W2912603959 hasAuthorship W2912603959A5037504477 @default.
• W2912603959 hasAuthorship W2912603959A5051600714 @default.
• W2912603959 hasAuthorship W2912603959A5085984026 @default.
• W2912603959 hasBestOaLocation W29126039592 @default.
• W2912603959 hasConcept C101762097 @default.
• W2912603959 hasConcept C104317684 @default.
• W2912603959 hasConcept C150194340 @default.
• W2912603959 hasConcept C153911025 @default.
• W2912603959 hasConcept C181199279 @default.
• W2912603959 hasConcept C27153228 @default.
• W2912603959 hasConcept C2779697368 @default.
• W2912603959 hasConcept C2779703480 @default.
• W2912603959 hasConcept C2779732469 @default.
• W2912603959 hasConcept C2781372317 @default.
• W2912603959 hasConcept C40767141 @default.
• W2912603959 hasConcept C55493867 @default.
• W2912603959 hasConcept C86803240 @default.
• W2912603959 hasConceptScore W2912603959C101762097 @default.
• W2912603959 hasConceptScore W2912603959C104317684 @default.
• W2912603959 hasConceptScore W2912603959C150194340 @default.
• W2912603959 hasConceptScore W2912603959C153911025 @default.
• W2912603959 hasConceptScore W2912603959C181199279 @default.
• W2912603959 hasConceptScore W2912603959C27153228 @default.
• W2912603959 hasConceptScore W2912603959C2779697368 @default.
• W2912603959 hasConceptScore W2912603959C2779703480 @default.
• W2912603959 hasConceptScore W2912603959C2779732469 @default.
• W2912603959 hasConceptScore W2912603959C2781372317 @default.
• W2912603959 hasConceptScore W2912603959C40767141 @default.
• W2912603959 hasConceptScore W2912603959C55493867 @default.
• W2912603959 hasConceptScore W2912603959C86803240 @default.
• W2912603959 hasFunder F4320321505 @default.
• W2912603959 hasFunder F4320321837 @default.
• W2912603959 hasIssue "7" @default.
• W2912603959 hasLocation W29126039591 @default.
• W2912603959 hasLocation W29126039592 @default.
• W2912603959 hasLocation W29126039593 @default.
• W2912603959 hasOpenAccess W2912603959 @default.

• next