FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912608166> ?p ?o ?g. }

• W2912608166 abstract "The AVR4 effector, secreted by Cladosporium fulvum, has been demonstrated to be involved in pathogen virulence. Recent studies further demonstrated that Avr4 is highly conserved among several Cercospora species, indicating a potential important role of this gene in fungal virulence. Therefore, investigation to determine whether this fungal effector gene is present in Cercospora cf. flagellaris (previously known as C. kikuchii), the causal agent of soybean cercospora leaf blight (CLB) disease, and whether it plays any role in CLB disease development, is of great interest. In the present study, the Avr4 gene from C. cf. flagellaris was cloned and mutants lacking the expression of Avr4 were created by homologous recombination to investigate its role in fungal virulence, cercosporin production as well as CLB disease development. The ∆avr4 mutants produced little or no cercosporin in vitro and the mutants also had significantly reduced cercosporin toxin biosynthesis genes expression. Furthermore, ∆avr4 mutants grew faster and were more sensitive to chitinase in vitro than the wild type. Surprisingly, cercosporin could also directly suppress chitinolytic activity in vitro. When inoculated onto detached soybean leaves, these mutants exhibited reduced virulence compared to the wild type and no cercosporin was detected in mutant inoculated soybean leaves. Taken all together, the results suggest that AVR4 may contribute to the virulence of C. cf. flagellaris on soybean through protecting fungal hyphae and regulating cercosporin biosynthesis. Considering the importance of AVR4 in C. cf. flagellaris virulence, we selected a region of this gene for targeted gene suppression through host induced gene silencing (HIGS) to determine whether this can reduce CLB disease in soybean. It was found that HIGS plants carrying the BPMV-Avr4 construct showed less disease symptoms compared to control plants, and the reduction of symptoms was positively correlated with reduction in Avr4 transcript levels and fungal growth. To the best of our knowledge, this is the first study demonstrating the involvement of AVR4 in CLB disease development possibly through regulating cercosporin production as well as protecting fungal hyphae. In addition, this study also showed the potential of using HIGS as a tool to control this important disease of soybean" @default.
• W2912608166 created "2019-02-21" @default.
• W2912608166 creator A5077622229 @default.
• W2912608166 date "2022-06-10" @default.
• W2912608166 modified "2023-10-15" @default.
• W2912608166 title "The roles of AVR4 in fungal virulence,cercosporin biosynthesis and its potential use in host induced gene silencing for controlling cercospora leaf blight disease of soybeans" @default.
• W2912608166 cites W128291326 @default.
• W2912608166 cites W1532568719 @default.
• W2912608166 cites W1569317193 @default.
• W2912608166 cites W1614310946 @default.
• W2912608166 cites W1625002266 @default.
• W2912608166 cites W1647075334 @default.
• W2912608166 cites W1651381644 @default.
• W2912608166 cites W167184130 @default.
• W2912608166 cites W1677498178 @default.
• W2912608166 cites W1815784261 @default.
• W2912608166 cites W1839095954 @default.
• W2912608166 cites W1883664804 @default.
• W2912608166 cites W1893085474 @default.
• W2912608166 cites W190954123 @default.
• W2912608166 cites W1922903995 @default.
• W2912608166 cites W1936085961 @default.
• W2912608166 cites W1966381160 @default.
• W2912608166 cites W1967276739 @default.
• W2912608166 cites W1967484381 @default.
• W2912608166 cites W1970115015 @default.
• W2912608166 cites W1974776206 @default.
• W2912608166 cites W1976969980 @default.
• W2912608166 cites W1977308438 @default.
• W2912608166 cites W1979786907 @default.
• W2912608166 cites W1980310946 @default.
• W2912608166 cites W1981607645 @default.
• W2912608166 cites W1982546103 @default.
• W2912608166 cites W1983701638 @default.
• W2912608166 cites W1984711465 @default.
• W2912608166 cites W1987357838 @default.
• W2912608166 cites W1988042249 @default.
• W2912608166 cites W1988492560 @default.
• W2912608166 cites W1993946088 @default.
• W2912608166 cites W1999689775 @default.
• W2912608166 cites W2001192592 @default.
• W2912608166 cites W2003157641 @default.
• W2912608166 cites W2003388251 @default.
• W2912608166 cites W2006802793 @default.
• W2912608166 cites W2011165627 @default.
• W2912608166 cites W2012986985 @default.
• W2912608166 cites W2013642237 @default.
• W2912608166 cites W2014610793 @default.
• W2912608166 cites W2014820623 @default.
• W2912608166 cites W2019083941 @default.
• W2912608166 cites W2019777898 @default.
• W2912608166 cites W2022187444 @default.
• W2912608166 cites W2024495671 @default.
• W2912608166 cites W2025331640 @default.
• W2912608166 cites W2028952545 @default.
• W2912608166 cites W2029217472 @default.
• W2912608166 cites W2033386770 @default.
• W2912608166 cites W2034159501 @default.
• W2912608166 cites W2034877976 @default.
• W2912608166 cites W2034940458 @default.
• W2912608166 cites W2035465685 @default.
• W2912608166 cites W2038096432 @default.
• W2912608166 cites W2039123683 @default.
• W2912608166 cites W2047195096 @default.
• W2912608166 cites W2047332529 @default.
• W2912608166 cites W2047406017 @default.
• W2912608166 cites W2051026766 @default.
• W2912608166 cites W2051734428 @default.
• W2912608166 cites W2054565267 @default.
• W2912608166 cites W2055391618 @default.
• W2912608166 cites W2062115572 @default.
• W2912608166 cites W2063170388 @default.
• W2912608166 cites W2063301185 @default.
• W2912608166 cites W2064158484 @default.
• W2912608166 cites W2064773460 @default.
• W2912608166 cites W2065469815 @default.
• W2912608166 cites W2067788689 @default.
• W2912608166 cites W2068521300 @default.
• W2912608166 cites W2073538301 @default.
• W2912608166 cites W2075785656 @default.
• W2912608166 cites W2076890694 @default.
• W2912608166 cites W2076946393 @default.
• W2912608166 cites W2096245314 @default.
• W2912608166 cites W2097001357 @default.
• W2912608166 cites W2097617743 @default.
• W2912608166 cites W2098270943 @default.
• W2912608166 cites W2098539472 @default.
• W2912608166 cites W2102089668 @default.
• W2912608166 cites W2102967742 @default.
• W2912608166 cites W2104026923 @default.
• W2912608166 cites W2104161044 @default.
• W2912608166 cites W2105909024 @default.
• W2912608166 cites W2108244474 @default.
• W2912608166 cites W2109219143 @default.
• W2912608166 cites W2111540332 @default.
• W2912608166 cites W2112167962 @default.
• W2912608166 cites W2114570899 @default.
• W2912608166 cites W2115627744 @default.
• W2912608166 cites W2116004277 @default.
• W2912608166 cites W2118825215 @default.

• next