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• W2912608563 startingPage "1964" @default.
• W2912608563 abstract "Deamidation is an important degradation pathway for proteins. Estimating deamidation propensities is essential for predicting their long-term stability. However, predicting deamidation rates in folded proteins is challenging because higher-order structure has a significant and unpredictable effect on deamidation. Here, we investigated the correlation between amide hydrogen exchange (HX) and deamidation to assess the potential of using hydrogen exchange–mass spectrometry (HX-MS) to rapidly predict deamidation propensity. Maltose-binding protein and a structurally less stable mutant, W169G, were stored in the dark at pH 7.0 at 23 ± 2°C for 1 year. Deamidation at each asparagine site was measured using liquid chromatography–mass spectrometry after trypsin digestion. Deamidation rates at each deamidation site were determined based on first-order kinetics. HX rates at the deamidation sites were determined before storage using the shortest peptic peptide containing each site using conventional bottom-up HX-MS at pD 7.0 at 25°C. We observed a power law correlation between deamidation half-life and HX half-life for the NG sites with measurable kinetics. For NA sites, slow deamidation was only observed at 2 sites located in rapidly exchanging regions. Our findings demonstrate that HX-MS can be used to reliably and rapidly rank deamidation propensity in folded proteins." @default.
• W2912608563 created "2019-02-21" @default.
• W2912608563 creator A5018966281 @default.
• W2912608563 creator A5026266291 @default.
• W2912608563 creator A5069158601 @default.
• W2912608563 date "2019-06-01" @default.
• W2912608563 modified "2023-09-27" @default.
• W2912608563 title "Rapid Prediction of Deamidation Rates of Proteins to Assess Their Long-Term Stability Using Hydrogen Exchange–Mass Spectrometry" @default.
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• W2912608563 cites W1974900044 @default.
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• W2912608563 doi "https://doi.org/10.1016/j.xphs.2019.01.019" @default.
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