FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912609294> ?p ?o ?g. }

• W2912609294 abstract "Reproductive containment in farmed fish is highly desired for sustainable aquaculture to prevent genetic introgression with wild conspecifics and enhance productivity by suppressing sexual maturation. A number of strategies have already been implemented or have been tested in commercially important fish (e.g. triploidy, monosexing, hormonal therapies); however, they either do not result in 100% containment, or they cannot be applied to all species. One promising new approach consists in disrupting primordial germ cells (PGCs), at the origin of germline cells, to induce sterility. The work carried out in this doctoral thesis aimed to investigate the genes involved in the survival of germ cells and subsequently conduct a functional analysis of candidate genes using CRISPR/Cas9 gene editing system to ultimately provide the basis for the development of a novel sterilisation technique. Nile tilapia was chosen as the experimental animal as it is a major aquaculture species worldwide and the control of reproduction plays a critical role in the farming productivity in this species. In addition, the species has clear advantages as its whole genome sequence is accessible, the generation time is relatively short and zygotes can be available all year round. Initially, a panel of 11 candidate genes with reported roles in survival of PGCs was investigated during the ontogenic development which led to the selection of piwi-like (piwil) gene as a target for genome editing. Then, high temperature was tested as a means to induce germ cell loss to better understand the mechanism underlying germ cell survival and apoptosis, and this study confirmed the functional importance of piwil genes in relation to germ cell loss and proliferation. In addition, the study suggested potential subfunctionalisation within the Bcl-2 gene family which requires further investigation. The next step aimed to optimise the CRISPR/Cas9 gene editing method by improving the microinjection system and testing different concentrations of sgRNAs. Over 95% of injected embryos showed on-target mutation in piwil2 via zygote injection of CRISPR/Cas9 reagents and complete KO larvae were shown in half of the mutants, producing putative sterile fish. However, there was no clear association between the phenotypes in PGCs and the mutation rate. Further comparative studies of mutant screening methods including T7E1, RGEN, HRMA, fragment analysis and NGS revealed that the genotypes of F0 are highly mosaic, suggesting that deep sequencing is recommended for accurate and high throughput F0 screening and further improvement for predictable genome editing is required for a reliable gene functional analysis in F0. In summary, the current thesis provided new scientific knowledge and supporting evidence for the use of the CRISPR/Cas9 gene editing platform to study gene function associated with sterility, with the ultimate goal to develop an alternative sterilisation method in fish." @default.
• W2912609294 created "2019-02-21" @default.
• W2912609294 creator A5023540658 @default.
• W2912609294 date "2018-09-01" @default.
• W2912609294 modified "2023-09-27" @default.
• W2912609294 title "Research towards the effective disruption of reproductive competence in Nile tilapia Oreochromis niloticus" @default.
• W2912609294 cites W1997194352 @default.
• W2912609294 cites W2008205228 @default.
• W2912609294 cites W2031734913 @default.
• W2912609294 cites W2060179476 @default.
• W2912609294 cites W2298105180 @default.
• W2912609294 cites W2565555627 @default.
• W2912609294 cites W2613409938 @default.
• W2912609294 cites W2754584669 @default.
• W2912609294 cites W2806691969 @default.
• W2912609294 cites W1599764421 @default.
• W2912609294 hasPublicationYear "2018" @default.
• W2912609294 type Work @default.
• W2912609294 sameAs 2912609294 @default.
• W2912609294 citedByCount "0" @default.
• W2912609294 crossrefType "dissertation" @default.
• W2912609294 hasAuthorship W2912609294A5023540658 @default.
• W2912609294 hasConcept C104317684 @default.
• W2912609294 hasConcept C109825262 @default.
• W2912609294 hasConcept C110175623 @default.
• W2912609294 hasConcept C150903083 @default.
• W2912609294 hasConcept C2776687438 @default.
• W2912609294 hasConcept C2778658382 @default.
• W2912609294 hasConcept C2779122487 @default.
• W2912609294 hasConcept C2780129523 @default.
• W2912609294 hasConcept C2909208804 @default.
• W2912609294 hasConcept C505870484 @default.
• W2912609294 hasConcept C54355233 @default.
• W2912609294 hasConcept C69991583 @default.
• W2912609294 hasConcept C78458016 @default.
• W2912609294 hasConcept C8182607 @default.
• W2912609294 hasConcept C86803240 @default.
• W2912609294 hasConcept C86909935 @default.
• W2912609294 hasConcept C98108389 @default.
• W2912609294 hasConceptScore W2912609294C104317684 @default.
• W2912609294 hasConceptScore W2912609294C109825262 @default.
• W2912609294 hasConceptScore W2912609294C110175623 @default.
• W2912609294 hasConceptScore W2912609294C150903083 @default.
• W2912609294 hasConceptScore W2912609294C2776687438 @default.
• W2912609294 hasConceptScore W2912609294C2778658382 @default.
• W2912609294 hasConceptScore W2912609294C2779122487 @default.
• W2912609294 hasConceptScore W2912609294C2780129523 @default.
• W2912609294 hasConceptScore W2912609294C2909208804 @default.
• W2912609294 hasConceptScore W2912609294C505870484 @default.
• W2912609294 hasConceptScore W2912609294C54355233 @default.
• W2912609294 hasConceptScore W2912609294C69991583 @default.
• W2912609294 hasConceptScore W2912609294C78458016 @default.
• W2912609294 hasConceptScore W2912609294C8182607 @default.
• W2912609294 hasConceptScore W2912609294C86803240 @default.
• W2912609294 hasConceptScore W2912609294C86909935 @default.
• W2912609294 hasConceptScore W2912609294C98108389 @default.
• W2912609294 hasLocation W29126092941 @default.
• W2912609294 hasOpenAccess W2912609294 @default.
• W2912609294 hasPrimaryLocation W29126092941 @default.
• W2912609294 hasRelatedWork W1993682304 @default.
• W2912609294 hasRelatedWork W1994233822 @default.
• W2912609294 hasRelatedWork W2022822215 @default.
• W2912609294 hasRelatedWork W2023935959 @default.
• W2912609294 hasRelatedWork W2045914152 @default.
• W2912609294 hasRelatedWork W2120149603 @default.
• W2912609294 hasRelatedWork W2127954649 @default.
• W2912609294 hasRelatedWork W2139863005 @default.
• W2912609294 hasRelatedWork W2284202178 @default.
• W2912609294 hasRelatedWork W2293709496 @default.
• W2912609294 hasRelatedWork W2298105180 @default.
• W2912609294 hasRelatedWork W2301846236 @default.
• W2912609294 hasRelatedWork W2321865275 @default.
• W2912609294 hasRelatedWork W2436736166 @default.
• W2912609294 hasRelatedWork W2805377061 @default.
• W2912609294 hasRelatedWork W3012002441 @default.
• W2912609294 hasRelatedWork W3019848904 @default.
• W2912609294 hasRelatedWork W3035549251 @default.
• W2912609294 hasRelatedWork W3084535136 @default.
• W2912609294 hasRelatedWork W3165813304 @default.
• W2912609294 isParatext "false" @default.
• W2912609294 isRetracted "false" @default.
• W2912609294 magId "2912609294" @default.
• W2912609294 workType "dissertation" @default.