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• W2912636430 abstract "Purpose: Mayaro virus (MAYV) is an emerging alphavirus circulating in Central/South America. It is transmitted to humans by mosquito bites, causing a febrile illness often with prolonged joint inflammation which resembles other infections with co-circulating arboviruses e.g. Dengue (DENV), Chikungunya (CHIKV) or Oropouche virus (OROV). Serological differentiation from alphavirus infections is complicated by antibodies targeting homologous antigens from related viruses (Semliki Forest virus complex), primarily CHIKV. Still, antibody detection can expand the time for diagnostics of acute MAYV infections beyond the short viraemic period suitable for MAYV RNA detection. Here, serum samples of patients with MAYV or other arboviral infections have been analysed with a novel Anti-Mayaro-virus-ELISA for detection of specific IgM and IgG at the Instituto Evadro Chagas. Methods & Materials: Serum samples originate from Brazilian patients with clinically and serologically characterized febrile infections drawn between day 11 and 117 post symptom onset. Pre-Characterization included analyses for IgM and haemagglutination inhibition (IH) antibodies against MAYV, CHIKV, DENV, Yellow fever virus (YFV), Zika virus (ZIKV), OROV, Eastern and Western Equine Encephalomyelitis virus (EEEV and WEEV) and flaviviruses in general using in-house MAC ELISA and indirect haemagglutination inhibition assay (IHA). The first collective encompassed 46 samples, including 25 negative and 21 positive for anti-MAYV IgM and IH antibodies. The second collective (n = 12) consisted of 6 anti-MAYV IH antibody positive and 6 anti-MAYV IH antibody negative samples. Samples were investigated with the Anti-Mayaro-virus-ELISA (Euroimmun, Germany) IgM (collective 1) and IgG (collective 2). Results: The Anti-Mayaro-virus-ELISA IgM was 100% sensitive (21/21) and 76% specific (19/25). 6 samples with reactivity in the ELISA had been pre-characterized for CHIKV (n = 5/11) or general flavivirus (n = 1/3) infection. Testing collective 2, the Anti-Mayaro-virus-ELISA IgG revealed a sensitivity of 100% (6/6) at 50% specificity (3/6). 3 samples with anti-CHIKV IH antibody positive but anti-MAYV IH antibody negative pre-characterization were also reactive in the ELISA. Conclusion: The novel Anti-Mayaro-virus-ELISA IgM and IgG showed high sensitivity at moderate specificity. This specificity meets the expectations and, in the majority of cases, can be explained by the cross-reactivity with antibodies against related viruses, primarily CHIKV. Thus the ELISA are suitable as screening assays reliably detecting MAYV infected patients." @default.
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• W2912636430 date "2019-02-01" @default.
• W2912636430 modified "2023-10-18" @default.
• W2912636430 title "Novel screening ELISA for sensitive detection of Mayaro virus infected patients" @default.
• W2912636430 doi "https://doi.org/10.1016/j.ijid.2018.11.362" @default.
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