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• W2912647362 endingPage "711" @default.
• W2912647362 startingPage "711" @default.
• W2912647362 abstract "Neuron-glia interactions contribute to pain initiation and sustainment. Intra-ganglionic (IG) secretion of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) modulates pain transmission through neuron-glia signaling, contributing to various orofacial pain conditions. The present study aimed to investigate the role of satellite glial cells (SGC) in TG in causing cytokine-related orofacial nociception in response to IG administration of CGRP. For that purpose, CGRP alone (10 μL of 10−5 M), Minocycline (5 μL containing 10 μg) followed by CGRP with one hour gap (Min + CGRP) were administered directly inside the TG in independent experiments. Rats were evaluated for thermal hyperalgesia at 6 and 24 h post-injection using an operant orofacial pain assessment device (OPAD) at three temperatures (37, 45 and 10 °C). Quantitative real-time PCR was performed to evaluate the mRNA expression of IL-1β, IL-6, TNF-α, IL-1 receptor antagonist (IL-1RA), sodium channel 1.7 (NaV 1.7, for assessment of neuronal activation) and glial fibrillary acidic protein (GFAP, a marker of glial activation). The cytokines released in culture media from purified glial cells were evaluated using antibody cytokine array. IG CGRP caused heat hyperalgesia between 6–24 h (paired-t test, p < 0.05). Between 1 to 6 h the mRNA and protein expressions of GFAP was increased in parallel with an increase in the mRNA expression of pro-inflammatory cytokines IL-1β and anti-inflammatory cytokine IL-1RA and NaV1.7 (one-way ANOVA followed by Dunnett’s post hoc test, p < 0.05). To investigate whether glial inhibition is useful to prevent nociception symptoms, Minocycline (glial inhibitor) was administered IG 1 h before CGRP injection. Minocycline reversed CGRP-induced thermal nociception, glial activity, and down-regulated IL-1β and IL-6 cytokines significantly at 6 h (t-test, p < 0.05). Purified glial cells in culture showed an increase in release of 20 cytokines after stimulation with CGRP. Our findings demonstrate that SGCs in the sensory ganglia contribute to the occurrence of pain via cytokine expression and that glial inhibition can effectively control the development of nociception." @default.
• W2912647362 created "2019-02-21" @default.
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• W2912647362 date "2019-02-07" @default.
• W2912647362 modified "2023-09-30" @default.
• W2912647362 title "CGRP Induces Differential Regulation of Cytokines from Satellite Glial Cells in Trigeminal Ganglia and Orofacial Nociception" @default.
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• W2912647362 cites W1592535071 @default.
• W2912647362 cites W1899754108 @default.
• W2912647362 cites W1966481705 @default.
• W2912647362 cites W1969966561 @default.
• W2912647362 cites W1970846628 @default.
• W2912647362 cites W1973413068 @default.
• W2912647362 cites W1976091899 @default.
• W2912647362 cites W1977886861 @default.
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• W2912647362 cites W1991983514 @default.
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• W2912647362 cites W1995092933 @default.
• W2912647362 cites W1998787695 @default.
• W2912647362 cites W2004159332 @default.
• W2912647362 cites W2006193913 @default.
• W2912647362 cites W2013173477 @default.
• W2912647362 cites W2015249233 @default.
• W2912647362 cites W2015518484 @default.
• W2912647362 cites W2016448869 @default.
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• W2912647362 cites W2030621034 @default.
• W2912647362 cites W2038864055 @default.
• W2912647362 cites W2040158215 @default.
• W2912647362 cites W2043772697 @default.
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• W2912647362 cites W2049925066 @default.
• W2912647362 cites W2062688383 @default.
• W2912647362 cites W2073997846 @default.
• W2912647362 cites W2075715972 @default.
• W2912647362 cites W2077534408 @default.
• W2912647362 cites W2078028780 @default.
• W2912647362 cites W2078519585 @default.
• W2912647362 cites W2089386481 @default.
• W2912647362 cites W2098475244 @default.
• W2912647362 cites W2104082801 @default.
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• W2912647362 cites W2120591359 @default.
• W2912647362 cites W2124502026 @default.
• W2912647362 cites W2125726418 @default.
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• W2912647362 cites W2155770121 @default.
• W2912647362 cites W2156289861 @default.
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• W2912647362 cites W2514113696 @default.
• W2912647362 cites W2570330573 @default.
• W2912647362 cites W2580542880 @default.
• W2912647362 cites W2603334196 @default.
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• W2912647362 doi "https://doi.org/10.3390/ijms20030711" @default.
• W2912647362 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6386987" @default.
• W2912647362 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30736422" @default.
• W2912647362 hasPublicationYear "2019" @default.
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