Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912656946> ?p ?o ?g. }
- W2912656946 endingPage "567" @default.
- W2912656946 startingPage "550" @default.
- W2912656946 abstract "The cotton mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is a polyphagous insect that attacks tens of plant and causes substantial economic loss. Insect chitinases are required to remove the old cuticle to allow for continued growth and development. Though insect chitinases have been well studied in tens of insects, their functions in mealybug are still not addressed. Here, we sequenced the transcriptomes of adult males and females, from which eight chitinase genes were identified. We then used the method of rapid amplification of cDNA ends to amplify their full length. Phylogenetic analysis indicated that these genes clustered into five subgroups. Among which, group II PsCht2 had the longest transcript and was highly expressed at second instar nymph. PsCht10, PsCht3-3 and PsIDGF were highly expressed in the adult females, whereas PsCht4 and PsCht4-1 were significantly expressed at the male pupa and adult male. Next, we knocked down all eight chitinase genes by feeding the double-stranded RNA. Knockdown of PsCht4 or PsCht4-1 led to the failure of moult and, silencing PsCht5 resulted in pupation defect, while silencing PsCht10 led to small body size, suggesting these genes have essential roles in development and can be used as a potential target for pest control." @default.
- W2912656946 created "2019-02-21" @default.
- W2912656946 creator A5013734787 @default.
- W2912656946 creator A5030401649 @default.
- W2912656946 creator A5032943455 @default.
- W2912656946 creator A5041132154 @default.
- W2912656946 creator A5054250936 @default.
- W2912656946 creator A5070046461 @default.
- W2912656946 creator A5082196554 @default.
- W2912656946 creator A5086000682 @default.
- W2912656946 date "2019-02-25" @default.
- W2912656946 modified "2023-10-10" @default.
- W2912656946 title "The functional difference of eight chitinase genes between male and female of the cotton mealybug, <i>Phenacoccus solenopsis</i>" @default.
- W2912656946 cites W103430187 @default.
- W2912656946 cites W1515462601 @default.
- W2912656946 cites W1527301642 @default.
- W2912656946 cites W1549352522 @default.
- W2912656946 cites W1706498001 @default.
- W2912656946 cites W1718799863 @default.
- W2912656946 cites W1816295733 @default.
- W2912656946 cites W1963566235 @default.
- W2912656946 cites W1968383728 @default.
- W2912656946 cites W1978436066 @default.
- W2912656946 cites W1981469178 @default.
- W2912656946 cites W1987868742 @default.
- W2912656946 cites W1989344768 @default.
- W2912656946 cites W1990444531 @default.
- W2912656946 cites W1996399811 @default.
- W2912656946 cites W1999574084 @default.
- W2912656946 cites W2000751595 @default.
- W2912656946 cites W2001256177 @default.
- W2912656946 cites W2020051355 @default.
- W2912656946 cites W2020754199 @default.
- W2912656946 cites W2027366921 @default.
- W2912656946 cites W2035471199 @default.
- W2912656946 cites W2041822372 @default.
- W2912656946 cites W2061010072 @default.
- W2912656946 cites W2061515984 @default.
- W2912656946 cites W2065542901 @default.
- W2912656946 cites W2068818899 @default.
- W2912656946 cites W2069502744 @default.
- W2912656946 cites W2090745581 @default.
- W2912656946 cites W2094974659 @default.
- W2912656946 cites W2100766338 @default.
- W2912656946 cites W2107277218 @default.
- W2912656946 cites W2108276490 @default.
- W2912656946 cites W2108284976 @default.
- W2912656946 cites W2126098632 @default.
- W2912656946 cites W2126419817 @default.
- W2912656946 cites W2133431389 @default.
- W2912656946 cites W2137961859 @default.
- W2912656946 cites W2140542179 @default.
- W2912656946 cites W2151957037 @default.
- W2912656946 cites W2153471314 @default.
- W2912656946 cites W2179438025 @default.
- W2912656946 cites W2180072250 @default.
- W2912656946 cites W2204805509 @default.
- W2912656946 cites W2277248045 @default.
- W2912656946 cites W2279637600 @default.
- W2912656946 cites W2284863332 @default.
- W2912656946 cites W2311203695 @default.
- W2912656946 cites W2336673993 @default.
- W2912656946 cites W2338964806 @default.
- W2912656946 cites W2460334518 @default.
- W2912656946 cites W2525695094 @default.
- W2912656946 cites W2554775602 @default.
- W2912656946 cites W2559011563 @default.
- W2912656946 cites W2583020420 @default.
- W2912656946 cites W2589570095 @default.
- W2912656946 cites W2597324672 @default.
- W2912656946 cites W2773165768 @default.
- W2912656946 cites W2951257985 @default.
- W2912656946 cites W4250362238 @default.
- W2912656946 cites W4251776901 @default.
- W2912656946 cites W4322388508 @default.
- W2912656946 cites W567718216 @default.
- W2912656946 doi "https://doi.org/10.1111/imb.12572" @default.
- W2912656946 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30739379" @default.
- W2912656946 hasPublicationYear "2019" @default.
- W2912656946 type Work @default.
- W2912656946 sameAs 2912656946 @default.
- W2912656946 citedByCount "31" @default.
- W2912656946 countsByYear W29126569462018 @default.
- W2912656946 countsByYear W29126569462019 @default.
- W2912656946 countsByYear W29126569462020 @default.
- W2912656946 countsByYear W29126569462021 @default.
- W2912656946 countsByYear W29126569462022 @default.
- W2912656946 countsByYear W29126569462023 @default.
- W2912656946 crossrefType "journal-article" @default.
- W2912656946 hasAuthorship W2912656946A5013734787 @default.
- W2912656946 hasAuthorship W2912656946A5030401649 @default.
- W2912656946 hasAuthorship W2912656946A5032943455 @default.
- W2912656946 hasAuthorship W2912656946A5041132154 @default.
- W2912656946 hasAuthorship W2912656946A5054250936 @default.
- W2912656946 hasAuthorship W2912656946A5070046461 @default.
- W2912656946 hasAuthorship W2912656946A5082196554 @default.
- W2912656946 hasAuthorship W2912656946A5086000682 @default.
- W2912656946 hasConcept C104317684 @default.