FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912666525> ?p ?o ?g. }

• W2912666525 abstract "Abstract In pharmaceutical research, high-content screening is an integral part of lead candidate development. Drug response in vitro over 40 parameters including biomarkers, signaling molecules, cell morphological changes, proliferation indexes and toxicity in a single sample could significantly enhance discovery of new therapeutics. As a proof of concept, we present a workflow for multidimensional Imaging Mass Cytometry™ (IMC™) and data processing with open source computational tools. CellProfiler was used to identify single cells through establishing cellular boundaries, followed by histoCAT™ (histology topography cytometry analysis toolbox) for extracting single-cell quantitative information visualized as t-SNE plots and heatmaps. Human breast cancer-derived cell lines SKBR3, HCC1143 and MCF-7 were screened for expression of cellular markers to generate digital images with a resolution comparable to conventional fluorescence microscopy. Predicted pharmacodynamic effects were measured in MCF-7 cells dosed with three target-specific compounds: growth stimulatory EGF, microtubule depolymerization agent nocodazole and genotoxic chemotherapeutic drug etoposide. We show strong pairwise correlation between nuclear markers pHistone3 S28 , Ki-67 and p4E-BP1 T37/T46 in classified mitotic cells and anti-correlation with cell surface markers. Our study demonstrates that IMC data expands the number of measured parameters in single cells and brings higher-dimension analysis to the field of cell-based screening in early lead compound discovery." @default.
• W2912666525 created "2019-02-21" @default.
• W2912666525 creator A5006824850 @default.
• W2912666525 creator A5030733670 @default.
• W2912666525 creator A5087541423 @default.
• W2912666525 date "2019-02-18" @default.
• W2912666525 modified "2023-09-23" @default.
• W2912666525 title "Multidimensional Profiling of Drug-Treated Cells by Imaging Mass Cytometry" @default.
• W2912666525 cites W1531821806 @default.
• W2912666525 cites W1965659632 @default.
• W2912666525 cites W1985317603 @default.
• W2912666525 cites W1988706652 @default.
• W2912666525 cites W1991339921 @default.
• W2912666525 cites W1992648778 @default.
• W2912666525 cites W1994930469 @default.
• W2912666525 cites W2012744533 @default.
• W2912666525 cites W2018195928 @default.
• W2912666525 cites W2026548544 @default.
• W2912666525 cites W2038254572 @default.
• W2912666525 cites W2053129129 @default.
• W2912666525 cites W2060300932 @default.
• W2912666525 cites W2075438156 @default.
• W2912666525 cites W2086080480 @default.
• W2912666525 cites W2087312216 @default.
• W2912666525 cites W2104036010 @default.
• W2912666525 cites W2126273383 @default.
• W2912666525 cites W2130072062 @default.
• W2912666525 cites W2144963196 @default.
• W2912666525 cites W2147443169 @default.
• W2912666525 cites W2159457208 @default.
• W2912666525 cites W2160676466 @default.
• W2912666525 cites W2167841373 @default.
• W2912666525 cites W2168102484 @default.
• W2912666525 cites W2183389629 @default.
• W2912666525 cites W2198136410 @default.
• W2912666525 cites W2270142843 @default.
• W2912666525 cites W2272856212 @default.
• W2912666525 cites W2274554953 @default.
• W2912666525 cites W2334138351 @default.
• W2912666525 cites W2407075689 @default.
• W2912666525 cites W2461427403 @default.
• W2912666525 cites W2467414813 @default.
• W2912666525 cites W2471907651 @default.
• W2912666525 cites W2480007569 @default.
• W2912666525 cites W2515544675 @default.
• W2912666525 cites W2522830304 @default.
• W2912666525 cites W2548467431 @default.
• W2912666525 cites W2570033955 @default.
• W2912666525 cites W2584824328 @default.
• W2912666525 cites W2585179645 @default.
• W2912666525 cites W2612467560 @default.
• W2912666525 cites W2729684734 @default.
• W2912666525 cites W2743344672 @default.
• W2912666525 cites W2751625431 @default.
• W2912666525 cites W2778357085 @default.
• W2912666525 cites W2785880648 @default.
• W2912666525 cites W2794660702 @default.
• W2912666525 cites W2832881433 @default.
• W2912666525 cites W4237945832 @default.
• W2912666525 doi "https://doi.org/10.1101/549592" @default.
• W2912666525 hasPublicationYear "2019" @default.
• W2912666525 type Work @default.
• W2912666525 sameAs 2912666525 @default.
• W2912666525 citedByCount "0" @default.
• W2912666525 crossrefType "posted-content" @default.
• W2912666525 hasAuthorship W2912666525A5006824850 @default.
• W2912666525 hasAuthorship W2912666525A5030733670 @default.
• W2912666525 hasAuthorship W2912666525A5087541423 @default.
• W2912666525 hasBestOaLocation W29126665251 @default.
• W2912666525 hasConcept C104317684 @default.
• W2912666525 hasConcept C127716648 @default.
• W2912666525 hasConcept C142669718 @default.
• W2912666525 hasConcept C1491633281 @default.
• W2912666525 hasConcept C153911025 @default.
• W2912666525 hasConcept C157044486 @default.
• W2912666525 hasConcept C185592680 @default.
• W2912666525 hasConcept C2778015335 @default.
• W2912666525 hasConcept C2779363559 @default.
• W2912666525 hasConcept C2780339063 @default.
• W2912666525 hasConcept C553184892 @default.
• W2912666525 hasConcept C55493867 @default.
• W2912666525 hasConcept C60644358 @default.
• W2912666525 hasConcept C70721500 @default.
• W2912666525 hasConcept C74187038 @default.
• W2912666525 hasConcept C86803240 @default.
• W2912666525 hasConcept C93304396 @default.
• W2912666525 hasConcept C95444343 @default.
• W2912666525 hasConceptScore W2912666525C104317684 @default.
• W2912666525 hasConceptScore W2912666525C127716648 @default.
• W2912666525 hasConceptScore W2912666525C142669718 @default.
• W2912666525 hasConceptScore W2912666525C1491633281 @default.
• W2912666525 hasConceptScore W2912666525C153911025 @default.
• W2912666525 hasConceptScore W2912666525C157044486 @default.
• W2912666525 hasConceptScore W2912666525C185592680 @default.
• W2912666525 hasConceptScore W2912666525C2778015335 @default.
• W2912666525 hasConceptScore W2912666525C2779363559 @default.
• W2912666525 hasConceptScore W2912666525C2780339063 @default.
• W2912666525 hasConceptScore W2912666525C553184892 @default.
• W2912666525 hasConceptScore W2912666525C55493867 @default.
• W2912666525 hasConceptScore W2912666525C60644358 @default.

• next