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• W2912668282 abstract "Serratia marcescens is a bacterium with the ability to colonize several niches, including some eukaryotic hosts. S. marcescens have been recently found in the gut of hematophagous insects that act as vectors of parasites, such as Anopheles, Rhodnius, and Triatoma. While some S. marcescens strains have been reported as symbiotic or pathogenic to other insects, the role of S. marcescens populations from gut microbiota of Rhodnius prolixus, insect vector of Chagas' disease, remains unknown. Bacterial colonies from R. prolixus gut were isolated on BHI agar. After BOX-PCR fingerprinting, the genomic sequences of two isolates RPA1 and RPH1 were compared to others S. marcescens from NCBI database in other to estimate their evolutionary divergence. Trypanolytic activity in vitro of these two bacterial isolates against two Trypanosoma cruzi DM28c clone and Y strain was assessed by microscopy. In addition, in vivo detection of type VI secretion system (T6SS) gene expression was observed by RT-PCR. Comparative genomics of S. marcescens RPA1 and RPH1 reveals, besides plasmid presence and genomic islands, genes related to motility, attachment and quorum-sensing in both genomes while genes for urea hydrolysis and Type II secretion system were found only in RPA1 genome. In vitro trypanolytic activity of both S. marcescens strains was stronger in their stationary phases of growth than in their exponential ones. In this way, 65-70% and 85-90% of epimastigotes (Dm28c clone and Y strain, respectively) were lysed after incubation with RPA1 or RPH1 in stationary phase. Although in vivo T6SS transcripts have been observed up to 40 days after feeding with T. cruzi infected blood, neither morbidity nor mortality was observed in R. prolixus. In this report, we made available two trypanolytic S. marcescens strains from R. prolixus gut to the scientific community together with their genomic sequences. We described genomic features with the purpose of bringing new insights into the S. marcescens adaptations for colonization of the specific niche of triatomine guts. This study provides the basis for a better understanding of the role of S. marcescens in the microbiota of R. prolixus gut as a potential antagonist of T. cruzi in this complex system." @default.
• W2912668282 created "2019-02-21" @default.
• W2912668282 creator A5001049120 @default.
• W2912668282 creator A5001141749 @default.
• W2912668282 creator A5009941476 @default.
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• W2912668282 creator A5060216193 @default.
• W2912668282 creator A5062804916 @default.
• W2912668282 creator A5075487323 @default.
• W2912668282 date "2019-01-24" @default.
• W2912668282 modified "2023-09-26" @default.
• W2912668282 title "In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract" @default.
• W2912668282 cites W1498122156 @default.
• W2912668282 cites W1500132433 @default.
• W2912668282 cites W1602473171 @default.
• W2912668282 cites W176339377 @default.
• W2912668282 cites W179584273 @default.
• W2912668282 cites W1819081776 @default.
• W2912668282 cites W1896879970 @default.
• W2912668282 cites W1896987173 @default.
• W2912668282 cites W1907385644 @default.
• W2912668282 cites W1935018540 @default.
• W2912668282 cites W1941136445 @default.
• W2912668282 cites W1965833563 @default.
• W2912668282 cites W1967616836 @default.
• W2912668282 cites W1969655028 @default.
• W2912668282 cites W1972541583 @default.
• W2912668282 cites W1985370649 @default.
• W2912668282 cites W1986160449 @default.
• W2912668282 cites W1993334278 @default.
• W2912668282 cites W1996289967 @default.
• W2912668282 cites W1996858055 @default.
• W2912668282 cites W2003335298 @default.
• W2912668282 cites W2005980568 @default.
• W2912668282 cites W2009974259 @default.
• W2912668282 cites W2011375465 @default.
• W2912668282 cites W2013559792 @default.
• W2912668282 cites W2013887744 @default.
• W2912668282 cites W2016383287 @default.
• W2912668282 cites W2016692739 @default.
• W2912668282 cites W2017971345 @default.
• W2912668282 cites W2018815811 @default.
• W2912668282 cites W2018824421 @default.
• W2912668282 cites W2020117070 @default.
• W2912668282 cites W2022801002 @default.
• W2912668282 cites W2023078596 @default.
• W2912668282 cites W2024291109 @default.
• W2912668282 cites W2027322754 @default.
• W2912668282 cites W2027462600 @default.
• W2912668282 cites W2027541185 @default.
• W2912668282 cites W2032445368 @default.
• W2912668282 cites W2039224522 @default.
• W2912668282 cites W2042787365 @default.
• W2912668282 cites W2043077036 @default.
• W2912668282 cites W2053351064 @default.
• W2912668282 cites W2053606119 @default.
• W2912668282 cites W2064912407 @default.
• W2912668282 cites W2065546327 @default.
• W2912668282 cites W2071708833 @default.
• W2912668282 cites W2072192435 @default.
• W2912668282 cites W2076262945 @default.
• W2912668282 cites W2079891312 @default.
• W2912668282 cites W2083176001 @default.
• W2912668282 cites W2085740152 @default.
• W2912668282 cites W2088061321 @default.
• W2912668282 cites W2089700620 @default.
• W2912668282 cites W2097065022 @default.
• W2912668282 cites W2097979496 @default.
• W2912668282 cites W2100525093 @default.
• W2912668282 cites W2101521042 @default.
• W2912668282 cites W2113553633 @default.
• W2912668282 cites W2114674757 @default.
• W2912668282 cites W2119888547 @default.
• W2912668282 cites W2121183668 @default.
• W2912668282 cites W2123008175 @default.
• W2912668282 cites W2123712967 @default.
• W2912668282 cites W2125397336 @default.
• W2912668282 cites W2127862696 @default.
• W2912668282 cites W2130394726 @default.
• W2912668282 cites W2132277086 @default.
• W2912668282 cites W2136421090 @default.
• W2912668282 cites W2138267773 @default.
• W2912668282 cites W2139906780 @default.
• W2912668282 cites W2154766752 @default.
• W2912668282 cites W2155793145 @default.
• W2912668282 cites W2155933274 @default.
• W2912668282 cites W2157892575 @default.
• W2912668282 cites W2159412804 @default.
• W2912668282 cites W2160522424 @default.
• W2912668282 cites W2172105162 @default.
• W2912668282 cites W2286085286 @default.
• W2912668282 cites W2289072065 @default.
• W2912668282 cites W2289103020 @default.
• W2912668282 cites W2306963058 @default.
• W2912668282 cites W2319901721 @default.
• W2912668282 cites W2331417839 @default.
• W2912668282 cites W2340840827 @default.
• W2912668282 cites W2356986436 @default.
• W2912668282 cites W2460324897 @default.
• W2912668282 cites W2516559547 @default.

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