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- W2912681817 abstract "It has long been appreciated that cell membranes are heterogeneous on length-scales relevant to the protein and lipid interactions that comprise biochemical networks at the cell surface. Lipid bilayer membranes, including plasma membranes isolated from mammalian cells, are capable of separating into distinct liquid phases. It is proposed that fluctuations associated with these phases comprise one mode of protein organization at the cell surface. Recent work from the Veatch group has shown that several peptide markers of ordered phases in isolated giant plasma membrane vesicles (GPMVs) are also enriched in B cell receptor (BCR) clusters in living B lymphocytes imaged using super-resolution localization microscopy. Meanwhile, efforts in the Levental group have quantified the partitioning of a broad assortment of membrane anchored peptides between liquid-ordered and liquid-disordered phases in isolated GPMVs. Here, we join forces to quantify the correspondence between peptide partitioning with respect to membrane phases in GPMVs and BCR clusters in intact cells. To date, we observe a remarkable qualitative correspondence when comparing the behavior of a small panel of distinct peptides probed in each system: peptides which favor the liquid-ordered phase in GPMVs are enriched in BCR clusters, while peptides that prefer the liquid-disordered phase in GPMVs are depleted from BCR clusters. Ongoing work is expanding to include additional peptides in both systems. Overall, our studies demonstrate a correspondence between phases in GPMVs and phase-like domains surrounding BCR clusters in intact cells. This work extends our previous efforts to describe the local membrane environment that is the context for BCR signaling and suggests that partitioning in GPMVs is a valuable experimental tool for predicting domain composition in intact cellular membranes." @default.
- W2912681817 created "2019-02-21" @default.
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- W2912681817 date "2019-02-01" @default.
- W2912681817 modified "2023-09-30" @default.
- W2912681817 title "Phase Partitioning of Peptide Anchors in Plasma Membrane Vesicles Predicts Their Recruitment to B Cell Receptor Clusters in Live Cells" @default.
- W2912681817 doi "https://doi.org/10.1016/j.bpj.2018.11.915" @default.
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