Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912689885> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2912689885 abstract "Abstract The ongoing evolution of microbial pathogens represents a significant issue in diagnostic PCR/qPCR. Many assays are burdened with false negativity due to mispriming and/or probe-binding failures. Therefore, PCR/qPCR assays used in the laboratory should be periodically re-assessed in silico on public sequences to evaluate the ability to detect actually circulating strains and to infer potentially escaping variants. In the work presented we re-assessed a RT-qPCR assay for the universal detection of influenza A (IA) viruses currently recommended by the European Union Reference Laboratory for Avian Influenza. To this end, the primers and probe sequences were challenged against more than 99,000 M-segment sequences in five data pools. To streamline this process, we developed a simple algorithm called the SequenceTracer designed for alignment stratification, compression, and personal sequence subset selection and also demonstrated its utility. The re-assessment confirmed the high inclusivity of the assay for the detection of avian, swine and human pandemic H1N1 IA viruses. On the other hand, the analysis identified human H3N2 strains with a critical probe-interfering mutation circulating since 2010, albeit with a significantly fluctuating proportion. Minor variations located in the forward and reverse primers identified in the avian and swine data were also considered." @default.
- W2912689885 created "2019-02-21" @default.
- W2912689885 creator A5029600207 @default.
- W2912689885 creator A5034506705 @default.
- W2912689885 creator A5047944177 @default.
- W2912689885 creator A5066274503 @default.
- W2912689885 creator A5066902434 @default.
- W2912689885 date "2019-02-07" @default.
- W2912689885 modified "2023-09-26" @default.
- W2912689885 title "In silico re-assessment of a diagnostic RT-qPCR assay for universal detection of Influenza A viruses" @default.
- W2912689885 cites W1904826643 @default.
- W2912689885 cites W1966272287 @default.
- W2912689885 cites W1986201353 @default.
- W2912689885 cites W1988533731 @default.
- W2912689885 cites W2003709801 @default.
- W2912689885 cites W2004234032 @default.
- W2912689885 cites W2005914654 @default.
- W2912689885 cites W2036496527 @default.
- W2912689885 cites W2048940051 @default.
- W2912689885 cites W2053741669 @default.
- W2912689885 cites W2069831575 @default.
- W2912689885 cites W2070721758 @default.
- W2912689885 cites W2094687107 @default.
- W2912689885 cites W2106306494 @default.
- W2912689885 cites W2124028672 @default.
- W2912689885 cites W2125875818 @default.
- W2912689885 cites W2126155530 @default.
- W2912689885 cites W2127043657 @default.
- W2912689885 cites W2127774996 @default.
- W2912689885 cites W2130946629 @default.
- W2912689885 cites W2159734253 @default.
- W2912689885 cites W2160378127 @default.
- W2912689885 cites W2170648607 @default.
- W2912689885 cites W2170964688 @default.
- W2912689885 cites W2335322902 @default.
- W2912689885 cites W2407348444 @default.
- W2912689885 cites W2418534182 @default.
- W2912689885 doi "https://doi.org/10.1038/s41598-018-37869-w" @default.
- W2912689885 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6367508" @default.
- W2912689885 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30733500" @default.
- W2912689885 hasPublicationYear "2019" @default.
- W2912689885 type Work @default.
- W2912689885 sameAs 2912689885 @default.
- W2912689885 citedByCount "15" @default.
- W2912689885 countsByYear W29126898852020 @default.
- W2912689885 countsByYear W29126898852021 @default.
- W2912689885 countsByYear W29126898852022 @default.
- W2912689885 countsByYear W29126898852023 @default.
- W2912689885 crossrefType "journal-article" @default.
- W2912689885 hasAuthorship W2912689885A5029600207 @default.
- W2912689885 hasAuthorship W2912689885A5034506705 @default.
- W2912689885 hasAuthorship W2912689885A5047944177 @default.
- W2912689885 hasAuthorship W2912689885A5066274503 @default.
- W2912689885 hasAuthorship W2912689885A5066902434 @default.
- W2912689885 hasBestOaLocation W29126898851 @default.
- W2912689885 hasConcept C104317684 @default.
- W2912689885 hasConcept C159047783 @default.
- W2912689885 hasConcept C2522874641 @default.
- W2912689885 hasConcept C2775905019 @default.
- W2912689885 hasConcept C2777546802 @default.
- W2912689885 hasConcept C3019819676 @default.
- W2912689885 hasConcept C519248777 @default.
- W2912689885 hasConcept C54355233 @default.
- W2912689885 hasConcept C70721500 @default.
- W2912689885 hasConcept C86803240 @default.
- W2912689885 hasConceptScore W2912689885C104317684 @default.
- W2912689885 hasConceptScore W2912689885C159047783 @default.
- W2912689885 hasConceptScore W2912689885C2522874641 @default.
- W2912689885 hasConceptScore W2912689885C2775905019 @default.
- W2912689885 hasConceptScore W2912689885C2777546802 @default.
- W2912689885 hasConceptScore W2912689885C3019819676 @default.
- W2912689885 hasConceptScore W2912689885C519248777 @default.
- W2912689885 hasConceptScore W2912689885C54355233 @default.
- W2912689885 hasConceptScore W2912689885C70721500 @default.
- W2912689885 hasConceptScore W2912689885C86803240 @default.
- W2912689885 hasIssue "1" @default.
- W2912689885 hasLocation W29126898851 @default.
- W2912689885 hasLocation W29126898852 @default.
- W2912689885 hasLocation W29126898853 @default.
- W2912689885 hasLocation W29126898854 @default.
- W2912689885 hasOpenAccess W2912689885 @default.
- W2912689885 hasPrimaryLocation W29126898851 @default.
- W2912689885 hasRelatedWork W1613633768 @default.
- W2912689885 hasRelatedWork W1713085160 @default.
- W2912689885 hasRelatedWork W2013135709 @default.
- W2912689885 hasRelatedWork W2144056263 @default.
- W2912689885 hasRelatedWork W2146797721 @default.
- W2912689885 hasRelatedWork W2147629250 @default.
- W2912689885 hasRelatedWork W2170463474 @default.
- W2912689885 hasRelatedWork W2330856237 @default.
- W2912689885 hasRelatedWork W2440528776 @default.
- W2912689885 hasRelatedWork W628000832 @default.
- W2912689885 hasVolume "9" @default.
- W2912689885 isParatext "false" @default.
- W2912689885 isRetracted "false" @default.
- W2912689885 magId "2912689885" @default.
- W2912689885 workType "article" @default.