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- W2912695452 abstract "Natural transformation contributes to acquisition and spread of antibiotic resistance and pathogenicity traits. We show that Bacillus subtilis chromosomal transformation decreased logarithmically with increased sequence divergence up to 15% in wild type (wt) cells or in cells lacking MutS2 or mismatch repair proteins (MutL, MutS or both). Beyond 15% sequence divergence, the chromosomal transformation efficiency is ~100-fold higher in mutS and mutSL than in mutS2 or wt cells. In the first phase of the biphasic curve (up to 15% sequence divergence), RecA-catalyzed DNA strand exchange contributes to the delineation of species, and in the second phase, homology-facilitated illegitimate recombination might facilitate the restoration of inactivated genes. To understand how MutSL modulates the integration process, we monitored DNA strand exchange reactions using a circular single-stranded DNA and a linear double-stranded DNA substrate with an internal 77-bp region with ~16% or ~54% sequence divergence in an otherwise homologous substrate. The former substrate delayed, whereas the latter halted RecA-mediated strand exchange. Interestingly, MutS addition overcame the heterologous barrier. We propose that MutS assists DNA strand exchange by facilitating RecA disassembly, and indirectly re-engagement with the homologous 5’-end of the linear duplex. Our data supports the idea that MutS modulates bidirectional RecA-mediated integration of divergent sequences, which is important for speciation without compromising genetic diversity" @default.
- W2912695452 created "2019-02-21" @default.
- W2912695452 creator A5005972848 @default.
- W2912695452 creator A5018736467 @default.
- W2912695452 creator A5034610855 @default.
- W2912695452 creator A5054597743 @default.
- W2912695452 creator A5084181476 @default.
- W2912695452 date "2019-02-13" @default.
- W2912695452 modified "2023-10-18" @default.
- W2912695452 title "Bacillus subtilis MutS Modulates RecA-Mediated DNA Strand Exchange Between Divergent DNA Sequences" @default.
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