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- W2912702342 abstract "Preparation instruments for scanning electron microscope (SEM) samples, such as critical point dryers and freeze dryers, are designed to controllably remove water from biological and similar liquid-based specimens. Cryo preparation techniques for scanning electron microscopy (cryo-SEM) are now considered essential for the successful observation of many wet or ‘beam sensitive’ specimens. There are essentially two methods of cooling the cold stage and anti-contaminator (cold trap) in the SEM chamber; braid cooling and cold nitrogen gas cooling. There are basically two choices when it comes to positioning the specimen preparation chamber: on column, where the chamber is bolted directly to the SEM, or off-column, where the preparation chamber is remote from the microscope. Carbon fibre is mainly used in electron microscopy (EM) to produce thin, electrically conductive coatings on specimens. Carbon fibre can be used for transmission electron microscopy (TEM) applications, but carbon rod is normally preferred due to superior control of the evaporation process." @default.
- W2912702342 created "2019-02-21" @default.
- W2912702342 creator A5023643586 @default.
- W2912702342 date "2019-02-15" @default.
- W2912702342 modified "2023-09-24" @default.
- W2912702342 title "SEM Cryo-Stages and Preparation Chambers" @default.
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- W2912702342 doi "https://doi.org/10.1002/9781118663233.ch8" @default.
- W2912702342 hasPublicationYear "2019" @default.
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