Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912732982> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2912732982 endingPage "201" @default.
- W2912732982 startingPage "195" @default.
- W2912732982 abstract "Pigs harbor several different species of mycoplasmas, of which Mycoplasma hyopneumoniae presents the most significant economic impact on the swine industry. While ELISAs are the predominant diagnostic assay to measure antibody responses during infection with M. hyopneumoniae, the assay itself is only a rough estimate of the total antibody response. It lends little information on pathogen-wide antigen-specific responses. In addition, antibody responses to M. hyopneumoniae as measured by ELISA are slow to develop in infected swine. Our goal was to determine if a protein microarray could be more sensitive and informative of the serological responses of pigs to M. hyopneumoniae infection. The gene sequences of approximately 50 M. hyopneumoniae surface proteins or protein fragments were cloned, mutated to remove UGA codons, expressed in Escherichia coli and purified. The arrays were used to interrogate pig sera from various sources. Sera from naturally-infected swine gave some variability in antigen-specific responses, but, unexpectedly, the responses against the C-terminal portion of the major adhesin P97 was weak in all animals, including those that were experimentally infected. In two of four 118-day experimentally-infected caesarian-derived colostrum-deprived pigs, the strongest antibody responses occurred on days 30 and 54 against members of the P97/P102 paralog families. Our Day 0 results in the other two animals indicate that although thought to be mycoplasma free by all known criteria (serology and PCR), they may have harbored an inapparent Mycoplasma infection. In summary, the protein microarray has the potential to identify new targets for assay development to enhance sensitivity of antibody-based assays." @default.
- W2912732982 created "2019-02-21" @default.
- W2912732982 creator A5004111995 @default.
- W2912732982 creator A5014987110 @default.
- W2912732982 creator A5015196800 @default.
- W2912732982 date "2019-03-01" @default.
- W2912732982 modified "2023-10-18" @default.
- W2912732982 title "Analysis of swine antigen-specific antibody responses to Mycoplasma hyopneumoniae infection determined by protein microarray" @default.
- W2912732982 cites W2006884793 @default.
- W2912732982 cites W2031334915 @default.
- W2912732982 cites W2036369997 @default.
- W2912732982 cites W2062422796 @default.
- W2912732982 cites W2063837943 @default.
- W2912732982 cites W2064491710 @default.
- W2912732982 cites W2065654861 @default.
- W2912732982 cites W2103171057 @default.
- W2912732982 cites W2106465866 @default.
- W2912732982 cites W2109998533 @default.
- W2912732982 cites W2145848654 @default.
- W2912732982 cites W2157056112 @default.
- W2912732982 cites W2253108680 @default.
- W2912732982 cites W2499264936 @default.
- W2912732982 doi "https://doi.org/10.1016/j.vetmic.2019.02.010" @default.
- W2912732982 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30827388" @default.
- W2912732982 hasPublicationYear "2019" @default.
- W2912732982 type Work @default.
- W2912732982 sameAs 2912732982 @default.
- W2912732982 citedByCount "6" @default.
- W2912732982 countsByYear W29127329822020 @default.
- W2912732982 countsByYear W29127329822021 @default.
- W2912732982 countsByYear W29127329822022 @default.
- W2912732982 countsByYear W29127329822023 @default.
- W2912732982 crossrefType "journal-article" @default.
- W2912732982 hasAuthorship W2912732982A5004111995 @default.
- W2912732982 hasAuthorship W2912732982A5014987110 @default.
- W2912732982 hasAuthorship W2912732982A5015196800 @default.
- W2912732982 hasConcept C104317684 @default.
- W2912732982 hasConcept C147483822 @default.
- W2912732982 hasConcept C150194340 @default.
- W2912732982 hasConcept C159047783 @default.
- W2912732982 hasConcept C159654299 @default.
- W2912732982 hasConcept C179470777 @default.
- W2912732982 hasConcept C186836561 @default.
- W2912732982 hasConcept C195814341 @default.
- W2912732982 hasConcept C203014093 @default.
- W2912732982 hasConcept C2775881110 @default.
- W2912732982 hasConcept C2776460866 @default.
- W2912732982 hasConcept C2777233064 @default.
- W2912732982 hasConcept C2778838687 @default.
- W2912732982 hasConcept C2780383596 @default.
- W2912732982 hasConcept C45189115 @default.
- W2912732982 hasConcept C54355233 @default.
- W2912732982 hasConcept C547475151 @default.
- W2912732982 hasConcept C86803240 @default.
- W2912732982 hasConcept C89423630 @default.
- W2912732982 hasConceptScore W2912732982C104317684 @default.
- W2912732982 hasConceptScore W2912732982C147483822 @default.
- W2912732982 hasConceptScore W2912732982C150194340 @default.
- W2912732982 hasConceptScore W2912732982C159047783 @default.
- W2912732982 hasConceptScore W2912732982C159654299 @default.
- W2912732982 hasConceptScore W2912732982C179470777 @default.
- W2912732982 hasConceptScore W2912732982C186836561 @default.
- W2912732982 hasConceptScore W2912732982C195814341 @default.
- W2912732982 hasConceptScore W2912732982C203014093 @default.
- W2912732982 hasConceptScore W2912732982C2775881110 @default.
- W2912732982 hasConceptScore W2912732982C2776460866 @default.
- W2912732982 hasConceptScore W2912732982C2777233064 @default.
- W2912732982 hasConceptScore W2912732982C2778838687 @default.
- W2912732982 hasConceptScore W2912732982C2780383596 @default.
- W2912732982 hasConceptScore W2912732982C45189115 @default.
- W2912732982 hasConceptScore W2912732982C54355233 @default.
- W2912732982 hasConceptScore W2912732982C547475151 @default.
- W2912732982 hasConceptScore W2912732982C86803240 @default.
- W2912732982 hasConceptScore W2912732982C89423630 @default.
- W2912732982 hasLocation W29127329821 @default.
- W2912732982 hasLocation W29127329822 @default.
- W2912732982 hasOpenAccess W2912732982 @default.
- W2912732982 hasPrimaryLocation W29127329821 @default.
- W2912732982 hasRelatedWork W1965987032 @default.
- W2912732982 hasRelatedWork W2016237685 @default.
- W2912732982 hasRelatedWork W2055685619 @default.
- W2912732982 hasRelatedWork W2124283227 @default.
- W2912732982 hasRelatedWork W2157713925 @default.
- W2912732982 hasRelatedWork W2370737338 @default.
- W2912732982 hasRelatedWork W2499264936 @default.
- W2912732982 hasRelatedWork W2565592345 @default.
- W2912732982 hasRelatedWork W2912732982 @default.
- W2912732982 hasRelatedWork W2945014939 @default.
- W2912732982 hasVolume "230" @default.
- W2912732982 isParatext "false" @default.
- W2912732982 isRetracted "false" @default.
- W2912732982 magId "2912732982" @default.
- W2912732982 workType "article" @default.