Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912757234> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2912757234 endingPage "91" @default.
- W2912757234 startingPage "87" @default.
- W2912757234 abstract "Summary In this paper, we propose a promising super‐resolution imaging scheme in fluorescence lifetime domain (lifetime super‐resolution optical fluctuation imaging, ltSOFI). ltSOFI has the potential to obtain super‐resolution images by taking advantage of fluorescence lifetime blinking under wide‐field lifetime detection. The proof‐of‐concept for ltSOFI was demonstrated through numerical simulation of high‐order cumulant analysis on fluorescence lifetime blinking emitters. As a tentative experimental demonstration, we obtained super‐resolution lifetime imaging from time‐lapse FLIM recording of HeLa cells expressing a cAMP sensor using ltSOFI method. ltSOFI is expected to initiate a new dimension in the lifetime domain for blinking‐based super‐resolution microscopy. Lay Description We report on a promising super‐resolution imaging scheme in fluorescence lifetime domain (lifetime super‐resolution optical fluctuation imaging, ltSOFI). ltSOFI has the potential to obtain super‐resolution images by taking advantage of fluorescence lifetime blinking under wide‐field lifetime detection. Past advances in super‐resolution fluorescence microscopy primarily rely on the spatiotemporal modulation of the fluorescence intensity. Although the applications of the Q‐dot blinking have been discussed in the literature, most of the discussions have focused on the blinking of fluorescence intensity. Few studies have shown the possibility of super‐resolution imaging through fluorescence lifetime fluctuations. In this paper, we proposed the ltSOFI scheme that explored the possibility of super‐resolution reconstruction from the blinking of fluorescence lifetime. The proof‐of‐concept for ltSOFI was demonstrated through numerical simulation of high‐order cumulant analysis on fluorescence lifetime blinking emitters. As a tentative experimental demonstration, we obtained super‐resolution lifetime imaging from time‐lapse FLIM recording of HeLa cells expressing a cAMP sensor using ltSOFI method. The ltSOFI method is expected to initiate a new dimension in the lifetime domain for blinking‐based super‐resolution microscopy. Moreover, the existing fluorescence lifetime imaging microscopy and super‐resolution nanoscopy can benefit from the implementation of ltSOFI to significantly improve the imaging spatial resolution of fluorescence lifetime images. In addition, the proof‐of‐concept demonstration achieved by the numerical simulation and tentative experiment will provide a new perspective for obtaining fluorescence lifetime images with much finer details." @default.
- W2912757234 created "2019-02-21" @default.
- W2912757234 creator A5032298471 @default.
- W2912757234 creator A5034445480 @default.
- W2912757234 creator A5054746272 @default.
- W2912757234 creator A5054826934 @default.
- W2912757234 date "2019-02-19" @default.
- W2912757234 modified "2023-10-16" @default.
- W2912757234 title "Lifetime super‐resolution optical fluctuation imaging" @default.
- W2912757234 cites W1965190141 @default.
- W2912757234 cites W1992858116 @default.
- W2912757234 cites W1995413508 @default.
- W2912757234 cites W2034518650 @default.
- W2912757234 cites W2051529542 @default.
- W2912757234 cites W2053270078 @default.
- W2912757234 cites W2054455698 @default.
- W2912757234 cites W2078799104 @default.
- W2912757234 cites W2092712606 @default.
- W2912757234 cites W2113146347 @default.
- W2912757234 cites W2117998556 @default.
- W2912757234 cites W2134396482 @default.
- W2912757234 cites W2142866567 @default.
- W2912757234 cites W2339403333 @default.
- W2912757234 cites W2339580774 @default.
- W2912757234 cites W2532379207 @default.
- W2912757234 cites W2554260837 @default.
- W2912757234 cites W2589983485 @default.
- W2912757234 cites W2788997417 @default.
- W2912757234 cites W2809481355 @default.
- W2912757234 cites W752928406 @default.
- W2912757234 doi "https://doi.org/10.1111/jmi.12786" @default.
- W2912757234 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30734939" @default.
- W2912757234 hasPublicationYear "2019" @default.
- W2912757234 type Work @default.
- W2912757234 sameAs 2912757234 @default.
- W2912757234 citedByCount "0" @default.
- W2912757234 crossrefType "journal-article" @default.
- W2912757234 hasAuthorship W2912757234A5032298471 @default.
- W2912757234 hasAuthorship W2912757234A5034445480 @default.
- W2912757234 hasAuthorship W2912757234A5054746272 @default.
- W2912757234 hasAuthorship W2912757234A5054826934 @default.
- W2912757234 hasConcept C120665830 @default.
- W2912757234 hasConcept C121332964 @default.
- W2912757234 hasConcept C138268822 @default.
- W2912757234 hasConcept C147080431 @default.
- W2912757234 hasConcept C154945302 @default.
- W2912757234 hasConcept C192562407 @default.
- W2912757234 hasConcept C205372480 @default.
- W2912757234 hasConcept C41008148 @default.
- W2912757234 hasConcept C57477423 @default.
- W2912757234 hasConcept C91881484 @default.
- W2912757234 hasConceptScore W2912757234C120665830 @default.
- W2912757234 hasConceptScore W2912757234C121332964 @default.
- W2912757234 hasConceptScore W2912757234C138268822 @default.
- W2912757234 hasConceptScore W2912757234C147080431 @default.
- W2912757234 hasConceptScore W2912757234C154945302 @default.
- W2912757234 hasConceptScore W2912757234C192562407 @default.
- W2912757234 hasConceptScore W2912757234C205372480 @default.
- W2912757234 hasConceptScore W2912757234C41008148 @default.
- W2912757234 hasConceptScore W2912757234C57477423 @default.
- W2912757234 hasConceptScore W2912757234C91881484 @default.
- W2912757234 hasFunder F4320321878 @default.
- W2912757234 hasIssue "2" @default.
- W2912757234 hasLocation W29127572341 @default.
- W2912757234 hasLocation W29127572342 @default.
- W2912757234 hasOpenAccess W2912757234 @default.
- W2912757234 hasPrimaryLocation W29127572341 @default.
- W2912757234 hasRelatedWork W1537873589 @default.
- W2912757234 hasRelatedWork W1996644878 @default.
- W2912757234 hasRelatedWork W2017666707 @default.
- W2912757234 hasRelatedWork W2086766692 @default.
- W2912757234 hasRelatedWork W2257225795 @default.
- W2912757234 hasRelatedWork W2296336181 @default.
- W2912757234 hasRelatedWork W2943569471 @default.
- W2912757234 hasRelatedWork W3003528977 @default.
- W2912757234 hasRelatedWork W3134642825 @default.
- W2912757234 hasRelatedWork W3135795858 @default.
- W2912757234 hasVolume "274" @default.
- W2912757234 isParatext "false" @default.
- W2912757234 isRetracted "false" @default.
- W2912757234 magId "2912757234" @default.
- W2912757234 workType "article" @default.