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- W2912785088 abstract "Treatment strategies for Alzheimer's disease (AD) include therapies for reducing amyloid deposits. To monitor effectiveness of these changes in living patients non–invasive surveillance is required. Radiolabelled compounds that can pass through the blood brain barrier and selectively bind to amyloid can be used to visualize deposits in the brain using PET, although these agents have not been used in a pre–clinical setting. Here we assess the staining characteristics of four putative PET ligands, using fluorescence microscopy and TASTPM mouse (APPswe × PS1.M146V) cortical sections containing amyloid deposits. Total amyloid load was assessed in 11 month old TASTPM mice using anti amyloid (peptide 13–27) immunohistochemistry and for β–amyloid peptide species using antibodies recognizing β–amyloid ×–40 and ×–42. Using a range of dilutions from 1mM to 1nM, the staining characteristics of PIB, BSB, SB–13 and FDDNP were compared to immunohistochemically labelled sections. On assessment of staining characteristics all ligands demonstrated plaques and vascular amyloid deposits to a similar level as total and ×–42 β–amyloid immunohistochemistry. At a concentration of 1uM, PIB and BSB clearly demonstrated whole amyloid deposits while SB–13 at a concentration of 10nM and to a greater extent FDDNP at 100nM clearly demonstrated mainly core plaque. BSB was the only ligand to show non–specific staining of elastic fibers in the vasculature down to a concentration of 10μM. These data highlight subtle differences between the ligands with respect to parenchymal and vascular plaque binding in the TASTPM mouse. Whether this reflects preference for specific amyloid species or the influence of the presence of rodent amyloid is the focus of ongoing studies." @default.
- W2912785088 created "2019-02-21" @default.
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- W2912785088 date "2006-07-01" @default.
- W2912785088 modified "2023-09-27" @default.
- W2912785088 title "P1-046: Assessment of putative positron emission tomography (PET) ligands on cortical sections from transgenic mice bearing amyloid precursor protein and presenillin-1 mutant transgenes (TASTPM)" @default.
- W2912785088 doi "https://doi.org/10.1016/j.jalz.2006.05.421" @default.
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