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- W2912787964 abstract "In recent years, gating and transient ion-pathway formation in the light-gated channelrhodopsins (ChRs) have been intensively studied. Despite these efforts, a profound understanding of the mechanistic details is still lacking. To track structural changes concomitant with the formation and subsequent collapse of the ion-conducting pore, we site-specifically introduced the artificial polarity-sensing probe p-azido-l-phenylalanine (azF) into several ChRs by amber stop codon suppression. The frequently used optogenetic actuator ReaChR (red-activatable ChR) exhibited the best expression properties of the wild type and the azF mutants. By exploiting the unique infrared spectral absorption of azF [νas(N3) ∼ 2100 cm-1] and its sensitivity to polarity changes, we monitored hydration changes at various sites of the pore region and the inner gate by stationary and time-resolved infrared spectroscopy. Our data imply that channel closure coincides with a dehydration event occurring between the interface of the central and the inner gate. In contrast, the extracellular ion pathway seems to be hydrated in the open and closed states to similar extents. Mutagenesis of sites in the inner gate suggests that it acts as an intracellular entry funnel, whose architecture and composition modulate water influx and efflux within the channel pore. Our results highlight the potential of genetic code expansion technology combined with biophysical methods to investigate channel gating, particularly hydration dynamics at specific sites, with a so far unprecedented spatial resolution." @default.
- W2912787964 created "2019-02-21" @default.
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- W2912787964 date "2019-01-31" @default.
- W2912787964 modified "2023-10-13" @default.
- W2912787964 title "Tracking Pore Hydration in Channelrhodopsin by Site-Directed Infrared-Active Azido Probes" @default.
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- W2912787964 doi "https://doi.org/10.1021/acs.biochem.8b01211" @default.
- W2912787964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30702875" @default.
- W2912787964 hasPublicationYear "2019" @default.
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