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- W2912796630 abstract "The human gut bacteriophage crAssphage has been proposed as a human-specific microbial source tracking (MST) marker for impacted water bodies. However, its global use as a human-specific MST marker requires validation in a tropical region. In this study, a crAssphage qPCR marker (CPQ_056) was detected in 21 sewage samples in Thailand with 100% sensitivity. The marker was detected in sewage from hospitals and residential buildings at 5.28–7.38 log10 copies/100 mL and in four influent and four effluent samples of municipal wastewater treatment plants at 4.23–6.19 and 3.78–4.89 log10 copies/100 mL, respectively. Furthermore, a 99.2% specificity (n = 127) was observed using feces from swine, cattle, chicken, duck, goat, sheep, buffalo, and fish, with cross-detection only occurring for one composite swine sample. The crAssphage marker was present in 56.25% (27 out of 48) of river samples at 3.20–7.29 log10 copies/100 mL. The concentrations of the crAssphage marker and a prevalidated human-specific Bacteroidales marker (HF183/BFDrev) did not differ significantly in any of the sewage or wastewater samples, whereas the crAssphage marker abundance was higher in river samples. This initial validation of the crAssphage gene as a human-specific MST marker in Southeast Asia will promote its inclusion in an MST toolbox." @default.
- W2912796630 created "2019-02-21" @default.
- W2912796630 creator A5020485469 @default.
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- W2912796630 date "2019-02-14" @default.
- W2912796630 modified "2023-10-10" @default.
- W2912796630 title "CrAssphage as a Potential Human Sewage Marker for Microbial Source Tracking in Southeast Asia" @default.
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- W2912796630 doi "https://doi.org/10.1021/acs.estlett.9b00041" @default.
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