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• W2912887428 abstract "Purpose: Brucella (B.) ovis is the causative agent of contagious ovine epididymitis a chronic disease in rams. Besides reduced fertility in breeding rams, it may cause infertility or abortion in ewes and increased perinatal mortality in lambs. Transmission occurs via passive venereal infection or direct ram-to-ram contact. Routine diagnostic is based on serological testing since clinical symptoms are often missing. In addition shedding of B. ovis is intermittent and a single negative sample does not guarantee freedom of disease. To study an association between seropositivity and shedding of B. ovis, three seropositive rams originating from a flock with 23 rams were transferred to the NRL for Bovine Brucellosis at the IVET Moedling for further investigations. Methods & Materials: The serological response and shedding of B. ovis were followed for four weeks. Blood samples were collected in weekly interval and tested with indirect enzyme-linked immunosorbent assay (I-ELISA) and complement-fixation test (CFT). Additionally, urine samples and preputial swabs were examined with bacteriological methods and real-time PCR. After the sampling period rams were diagnostically culled for pathological investigation. Results: None of the rams showed clinical signs by scrotal palpation. Two of the rams were seropositive in both serological tests, one ram only in I-ELISA. Brucella spp. could be identified by real-time PCR in urine samples of all rams and in preputial swabs of 2 rams at each sampling point. B.ovis was confirmed by bacteriological and genotyping methods. Gross morphological changes of the genital organs were detected in one animal only, displaying chronic periorchitis with adhesion between the parietal and visceral lamina of the tunica vaginalis, moderate scrotal serous effusion and bilateral thrombosis of testicular vessels. Conclusion: An association between B. ovis shedding and seropositivity was confirmed. Antibodies decreased weekly during the sample period, but shedding of B. ovis still remains. In-vivo diagnostic of preputial swabs and urine samples should be considered as an additional tool for direct diagnosis of B. ovis in serological positive rams. To prevent transmission, positive rams have to be separated from the flock and slaughtered. Our study showed that castration does not prevent spreading of the disease since shedding via urine is possible." @default.
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• W2912887428 date "2019-02-01" @default.
• W2912887428 modified "2023-09-26" @default.
• W2912887428 title "Isolation of B. ovis from preputial swabs and urine samples in subclinical infected rams" @default.
• W2912887428 doi "https://doi.org/10.1016/j.ijid.2018.11.178" @default.
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