FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912893695> ?p ?o ?g. }

• W2912893695 endingPage "1037" @default.
• W2912893695 startingPage "1020" @default.
• W2912893695 abstract "Stable recognition of the intron branchpoint (BP) by the U2 snRNP to form the pre-spliceosome is the first ATP-dependent step of splicing. Genetic and biochemical data from yeast indicate that Cus2 aids U2 snRNA folding into the stem IIa conformation prior to pre-spliceosome formation. Cus2 must then be removed by an ATP-dependent function of Prp5 before assembly can progress. However, the location from which Cus2 is displaced and the nature of its binding to the U2 snRNP are unknown. Here, we show that Cus2 contains a conserved UHM (U2AF homology motif) that binds Hsh155, the yeast homolog of human SF3b1, through a conserved ULM (U2AF ligand motif). Mutations in either motif block binding and allow pre-spliceosome formation without ATP. A 2.0 Å resolution structure of the Hsh155 ULM in complex with the UHM of Tat-SF1, the human homolog of Cus2, and complementary binding assays show that the interaction is highly similar between yeast and humans. Furthermore, we show that Tat-SF1 can replace Cus2 function by enforcing ATP dependence of pre-spliceosome formation in yeast extracts. Cus2 is removed before pre-spliceosome formation, and both Cus2 and its Hsh155 ULM binding site are absent from available cryo-EM structure models. However, our data are consistent with the apparent location of the disordered Hsh155 ULM between the U2 stem–loop IIa and the HEAT repeats of Hsh155 that interact with Prp5. We propose a model in which Prp5 uses ATP to remove Cus2 from Hsh155 such that extended base-pairing between U2 snRNA and the intron BP can occur." @default.
• W2912893695 created "2019-02-21" @default.
• W2912893695 creator A5002529484 @default.
• W2912893695 creator A5023625789 @default.
• W2912893695 creator A5041775986 @default.
• W2912893695 creator A5042097573 @default.
• W2912893695 creator A5045716192 @default.
• W2912893695 creator A5049964773 @default.
• W2912893695 creator A5060277224 @default.
• W2912893695 creator A5066477303 @default.
• W2912893695 creator A5070071808 @default.
• W2912893695 date "2019-05-20" @default.
• W2912893695 modified "2023-10-17" @default.
• W2912893695 title "Cus2 enforces the first ATP-dependent step of splicing by binding to yeast SF3b1 through a UHM–ULM interaction" @default.
• W2912893695 cites W1575542444 @default.
• W2912893695 cites W1594218135 @default.
• W2912893695 cites W1777520298 @default.
• W2912893695 cites W1884725878 @default.
• W2912893695 cites W1958447102 @default.
• W2912893695 cites W1972059985 @default.
• W2912893695 cites W1973010951 @default.
• W2912893695 cites W1973782344 @default.
• W2912893695 cites W1978612758 @default.
• W2912893695 cites W1990007398 @default.
• W2912893695 cites W1995460363 @default.
• W2912893695 cites W1996645650 @default.
• W2912893695 cites W2003277334 @default.
• W2912893695 cites W2008708467 @default.
• W2912893695 cites W2009221497 @default.
• W2912893695 cites W2020308159 @default.
• W2912893695 cites W2024267851 @default.
• W2912893695 cites W2034085554 @default.
• W2912893695 cites W2035350840 @default.
• W2912893695 cites W2045936068 @default.
• W2912893695 cites W2051537724 @default.
• W2912893695 cites W2055043387 @default.
• W2912893695 cites W2057190156 @default.
• W2912893695 cites W2063295008 @default.
• W2912893695 cites W2065572350 @default.
• W2912893695 cites W2065868028 @default.
• W2912893695 cites W2067000354 @default.
• W2912893695 cites W2068835587 @default.
• W2912893695 cites W2069368296 @default.
• W2912893695 cites W2073928054 @default.
• W2912893695 cites W2077592602 @default.
• W2912893695 cites W2094506799 @default.
• W2912893695 cites W2096024560 @default.
• W2912893695 cites W2097043404 @default.
• W2912893695 cites W2098189749 @default.
• W2912893695 cites W2098350092 @default.
• W2912893695 cites W2098594736 @default.
• W2912893695 cites W2103381064 @default.
• W2912893695 cites W2106290432 @default.
• W2912893695 cites W2106965934 @default.
• W2912893695 cites W2107026830 @default.
• W2912893695 cites W2107787782 @default.
• W2912893695 cites W2108921801 @default.
• W2912893695 cites W2109248504 @default.
• W2912893695 cites W2110211434 @default.
• W2912893695 cites W2115101918 @default.
• W2912893695 cites W2115420193 @default.
• W2912893695 cites W2116876551 @default.
• W2912893695 cites W2126409874 @default.
• W2912893695 cites W2127584949 @default.
• W2912893695 cites W2127843422 @default.
• W2912893695 cites W2142554423 @default.
• W2912893695 cites W2144081223 @default.
• W2912893695 cites W2147874841 @default.
• W2912893695 cites W2148393113 @default.
• W2912893695 cites W2148890785 @default.
• W2912893695 cites W2150099889 @default.
• W2912893695 cites W2153823864 @default.
• W2912893695 cites W2154046032 @default.
• W2912893695 cites W2158991904 @default.
• W2912893695 cites W2159509917 @default.
• W2912893695 cites W2159664316 @default.
• W2912893695 cites W2160065837 @default.
• W2912893695 cites W2160579210 @default.
• W2912893695 cites W2160683304 @default.
• W2912893695 cites W2163341755 @default.
• W2912893695 cites W2163686989 @default.
• W2912893695 cites W2163930559 @default.
• W2912893695 cites W2164351691 @default.
• W2912893695 cites W2166437079 @default.
• W2912893695 cites W2168915479 @default.
• W2912893695 cites W2180229411 @default.
• W2912893695 cites W2188899216 @default.
• W2912893695 cites W2193225046 @default.
• W2912893695 cites W2194238612 @default.
• W2912893695 cites W2413340136 @default.
• W2912893695 cites W2487288428 @default.
• W2912893695 cites W2489507000 @default.
• W2912893695 cites W2505052752 @default.
• W2912893695 cites W2518229206 @default.
• W2912893695 cites W2555946567 @default.
• W2912893695 cites W2560980003 @default.
• W2912893695 cites W2570514707 @default.
• W2912893695 cites W2573935228 @default.

• next