Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912930057> ?p ?o ?g. }
- W2912930057 endingPage "770" @default.
- W2912930057 startingPage "760" @default.
- W2912930057 abstract "The p53, p63 and p73 proteins belong to the p53 family of transcription factors, playing key roles in tumour suppression. The α-splice variant of p73 (p73α) has at its C terminus a sterile alpha motif (SAM); this domain, SAMp73, formed by five helices (α1 to α5), is thought to mediate in protein-protein interactions. The E3-ligase MDM2 binds to p73 at its N terminus transactivation domain (TA), but it does not promote its degradation via ubiquitination; however, the details of such MDM2/p73 interaction are not fully known.We studied the binding of SAMp73 with N-terminal MDM2, by several biophysical techniques, namely, fluorescence, far-UV circular dichroism (CD), NMR and bio-layer interferometry (BLI).Our results obtained by fluorescence, T2-relaxation measurements and BLI show that there was binding between both proteins with a dissociation constant of ~10 μM. Furthermore, the binding region of SAMp73 involved mainly residues in the major α-helix, α5, and the nearby α4, as shown by HSQC-NMR. The binding was so specific that an isolated peptide comprising α4 and α5 helices of SAMp73, α4α5, did also bind to the N terminus of MDM2, although with weaker affinity than the entire domain.A new interaction between MDM2 and SAMp73 has been found, which could have potential therapeutic applications in cancers involving inactivated p53.A novel interaction between the C-terminal SAM of p73 and N-terminal MDM2 is described. The interaction could be used to modulate the functions where the wild-type, intact p73 is involved." @default.
- W2912930057 created "2019-02-21" @default.
- W2912930057 creator A5032638676 @default.
- W2912930057 creator A5048872989 @default.
- W2912930057 creator A5065414460 @default.
- W2912930057 creator A5091231119 @default.
- W2912930057 date "2019-04-01" @default.
- W2912930057 modified "2023-10-18" @default.
- W2912930057 title "The C-terminal SAM domain of p73 binds to the N terminus of MDM2" @default.
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