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- W2912943937 abstract "Methods for microinjection into sea urchin eggs have become relatively easier because of the technical improvements by a number of researchers in the past decades. However, the size and the characteristics, such as the elasticity and toughness, of the eggs and embryos differ in species, so that we still need to modify the details of methods to adapt to each target. In this section, I list microinjection methods for three species: Hemicentrotus pulcherrimus, which has relatively tough eggs, Temnopleurus reevesii, which has slightly weak eggs, and Strongylocentrotus purpuratus, which is the most used species in sea urchin biology. In addition, I describe the methods for co-injection of morpholino anti-sense oligonucleotides and mRNAs, as well as the method for microinjection into blastomeres." @default.
- W2912943937 created "2019-02-21" @default.
- W2912943937 creator A5047898897 @default.
- W2912943937 date "2019-01-01" @default.
- W2912943937 modified "2023-09-27" @default.
- W2912943937 title "Microinjection methods for sea urchin eggs and blastomeres" @default.
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- W2912943937 doi "https://doi.org/10.1016/bs.mcb.2018.09.013" @default.
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