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• W2912972214 startingPage "035002" @default.
• W2912972214 abstract "The single-molecule pull-down (SiMPull) assay analyzes molecular complexes in physiological conditions from cell or tissue lysates. Currently the approach requires a lengthy sample preparation process, which has largely prevented the widespread adoption of this technique in bioanalysis. Here, we present a simplified SiMPull assay based upon dichlorodimethylsilane-Tween-20 passivation and F(ab) fragment labeling. Our passivation is a much shorter process than the standard polyethylene glycol passivation used in most single-molecule studies. The use of F(ab) fragments for indirect fluorescent labeling rather than divalent F(ab')2 or whole IgG antibodies allows for the pre-incubation of the detection antibodies, reducing the sample preparation time for single-molecule immunoprecipitation samples. We examine the applicability of our approach to recombinant proteins and endogenous proteins from mammalian cell lysates." @default.
• W2912972214 created "2019-02-21" @default.
• W2912972214 creator A5017630733 @default.
• W2912972214 creator A5032835106 @default.
• W2912972214 date "2019-03-22" @default.
• W2912972214 modified "2023-09-25" @default.
• W2912972214 title "Facile single-molecule pull-down assay for analysis of endogenous proteins" @default.
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• W2912972214 doi "https://doi.org/10.1088/1478-3975/ab0792" @default.
• W2912972214 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30769341" @default.
• W2912972214 hasPublicationYear "2019" @default.
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