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• W2912982498 abstract "The green alga Chlorella zofingiensis has been recognized as an industrially relevant strain because of its robust growth under multiple trophic conditions and the potential for simultaneous production of triacylglycerol (TAG) and the high-value keto-carotenoid astaxanthin. Nevertheless, the mechanism of TAG synthesis remains poorly understood in C. zofingiensis. Diacylglycerol acyltransferase (DGAT) is thought to catalyze the committed step of TAG assembly in the Kennedy pathway. C. zofingiensis genome is predicted to possess eleven putative DGAT-encoding genes, the greatest number ever found in green algae, pointing to the complexity of TAG assembly in the alga.The transcription start site of C. zofingiensis DGATs was determined by 5'-rapid amplification of cDNA ends (RACE), and their coding sequences were cloned and verified by sequencing, which identified ten DGAT genes (two type I DGATs designated as CzDGAT1A and CzDGAT1B, and eight type II DGATs designated as CzDGTT1 through CzDGTT8) and revealed that the previous gene models of seven DGATs were incorrect. Function complementation in the TAG-deficient yeast strain confirmed the functionality of most DGATs, with CzDGAT1A and CzDGTT5 having the highest activity. In vitro DGAT assay revealed that CzDGAT1A and CzDGTT5 preferred eukaryotic and prokaryotic diacylglycerols (DAGs), respectively, and had overlapping yet distinctive substrate specificity for acyl-CoAs. Subcellular co-localization experiment in tobacco leaves indicated that both CzDGAT1A and CzDGTT5 were localized at endoplasmic reticulum (ER). Upon nitrogen deprivation, TAG was drastically induced in C. zofingiensis, accompanied by a considerable up-regulation of CzDGAT1A and CzDGTT5. These two genes were probably regulated by the transcription factors (TFs) bZIP3 and MYB1, as suggested by the yeast one-hybrid assay and expression correlation. Moreover, heterologous expression of CzDGAT1A and CzDGTT5 promoted TAG accumulation and TAG yield in different hosts including yeast and oleaginous alga.Our study represents a pioneering work on the characterization of both type I and type II C. zofingiensis DGATs by systematically integrating functional complementation, in vitro enzymatic assay, subcellular localization, yeast one-hybrid assay and overexpression in yeast and oleaginous alga. These results (1) update the gene models of C. zofingiensis DGATs, (2) shed light on the mechanism of oleaginousness in which CzDGAT1A and CzDGTT5, have functional complementarity and probably work in collaboration at ER contributing to the abundance and complexity of TAG, and (3) provide engineering targets for future trait improvement via rational manipulation of this alga as well as other industrially relevant ones." @default.
• W2912982498 created "2019-02-21" @default.
• W2912982498 creator A5008666774 @default.
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• W2912982498 date "2019-02-11" @default.
• W2912982498 modified "2023-10-15" @default.
• W2912982498 title "Characterization of type I and type II diacylglycerol acyltransferases from the emerging model alga Chlorella zofingiensis reveals their functional complementarity and engineering potential" @default.
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• W2912982498 cites W17951829 @default.
• W2912982498 cites W1877938267 @default.
• W2912982498 cites W1949139897 @default.
• W2912982498 cites W1966008000 @default.
• W2912982498 cites W1969532000 @default.
• W2912982498 cites W1985119795 @default.
• W2912982498 cites W2004116758 @default.
• W2912982498 cites W2008463825 @default.
• W2912982498 cites W2019258796 @default.
• W2912982498 cites W2023255979 @default.
• W2912982498 cites W2029355147 @default.
• W2912982498 cites W2032396082 @default.
• W2912982498 cites W2042115934 @default.
• W2912982498 cites W2045842319 @default.
• W2912982498 cites W2047281856 @default.
• W2912982498 cites W2048513083 @default.
• W2912982498 cites W2051856081 @default.
• W2912982498 cites W2052225110 @default.
• W2912982498 cites W2052420635 @default.
• W2912982498 cites W2054793148 @default.
• W2912982498 cites W2056380302 @default.
• W2912982498 cites W2073671007 @default.
• W2912982498 cites W2075446141 @default.
• W2912982498 cites W2089275873 @default.
• W2912982498 cites W2089628542 @default.
• W2912982498 cites W2094758309 @default.
• W2912982498 cites W2102450255 @default.
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• W2912982498 cites W2103978564 @default.
• W2912982498 cites W2105537485 @default.
• W2912982498 cites W2106541027 @default.
• W2912982498 cites W2107848327 @default.
• W2912982498 cites W2120780572 @default.
• W2912982498 cites W2134586609 @default.
• W2912982498 cites W2135487705 @default.
• W2912982498 cites W2138192175 @default.
• W2912982498 cites W2141248661 @default.
• W2912982498 cites W2142168860 @default.
• W2912982498 cites W2152207030 @default.
• W2912982498 cites W2156778513 @default.
• W2912982498 cites W2157553436 @default.
• W2912982498 cites W2163357966 @default.
• W2912982498 cites W2164519838 @default.
• W2912982498 cites W2172006978 @default.
• W2912982498 cites W2181707686 @default.
• W2912982498 cites W2185208376 @default.
• W2912982498 cites W2194454073 @default.
• W2912982498 cites W2227572122 @default.
• W2912982498 cites W2258753467 @default.
• W2912982498 cites W2275355354 @default.
• W2912982498 cites W2284174360 @default.
• W2912982498 cites W2293486630 @default.
• W2912982498 cites W2346131033 @default.
• W2912982498 cites W2347049662 @default.
• W2912982498 cites W2443313574 @default.
• W2912982498 cites W2461927112 @default.
• W2912982498 cites W2509711941 @default.
• W2912982498 cites W2561533418 @default.
• W2912982498 cites W2589372186 @default.
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• W2912982498 cites W3204631443 @default.
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• W2912982498 doi "https://doi.org/10.1186/s13068-019-1366-2" @default.
• W2912982498 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6371474" @default.
• W2912982498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30792816" @default.
• W2912982498 hasPublicationYear "2019" @default.
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