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• W2912982582 endingPage "227" @default.
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• W2912982582 abstract "HLA class I molecules are highly polymorphic cell surface proteins that trigger immune responses by CD8+ T cells and natural killer (NK) cells. Most humans express six different HLA class I proteins encoded by the HLA-A, HLA-B and HLA-C genes. HLA class I molecules bind to peptide antigens and present these antigens to T cell receptors (TCR) of CD8+ T cells. HLA class I expression levels also regulate NK cell activation. The presence of individual HLA class I genes is linked to many different disease, transplantation and therapy outcomes. An understanding of HLA class I expression and stability patterns is fundamentally important towards a better understanding of the associations of HLA class I genes with disease and treatment outcomes, and towards HLA class I targeting for vaccine development. Quantitative flow cytometry allows for assessments of variations in expression levels of HLA class I molecules in cells from a single blood donor over time, as well as averaged measurements across donors for the same allotype. Since all HLA class I molecules are structurally-related, cellular measurements of the HLA class I expression levels and stabilities of individual variants in human cells require careful choices of donors and antibodies, which are discussed here." @default.
• W2912982582 created "2019-02-21" @default.
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• W2912982582 date "2019-04-01" @default.
• W2912982582 modified "2023-10-18" @default.
• W2912982582 title "Strategies for the measurements of expression levels and half-lives of HLA class I allotypes" @default.
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• W2912982582 doi "https://doi.org/10.1016/j.humimm.2019.02.001" @default.
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