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• W2912984553 startingPage "513" @default.
• W2912984553 abstract "Abstract The minor capsid protein L2 of papillomaviruses exhibits multiple functions during viral entry including membrane interaction. Information on the protein is scarce, because of its high tendency of aggregation. We determined suitable conditions to produce a functional human papillomavirus (HPV) 16 L2 protein and thereby provide the opportunity for extensive in vitro analysis with respect to structural and biochemical information on L2 proteins and mechanistic details in viral entry. We produced the L2 protein of high-risk HPV 16 in Escherichia coli as inclusion bodies and purified the protein under denaturing conditions. A successive buffer screen resulted in suitable conditions for the biophysical characterization of 16L2. Analytical ultracentrifugation of the refolded protein showed a homogenous monomeric species. Furthermore, refolded 16L2 shows secondary structure elements. The N-terminal region including the proposed transmembrane region of 16L2 shows alpha-helical characteristics. However, overall 16L2 appears largely unstructured. Refolded 16L2 is capable of binding to DNA indicating that the putative DNA-binding regions are accessible in refolded 16L2. Further the refolded protein interacts with liposomal membranes presumably via the proposed transmembrane region at neutral pH without structural changes. This indicates that 16L2 can initially interact with membranes via pre-existing structural features." @default.
• W2912984553 created "2019-02-21" @default.
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• W2912984553 date "2018-10-30" @default.
• W2912984553 modified "2023-10-02" @default.
• W2912984553 title "Refolding and <i>in vitro</i> characterization of human papillomavirus 16 minor capsid protein L2" @default.
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• W2912984553 doi "https://doi.org/10.1515/hsz-2018-0311" @default.
• W2912984553 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30375341" @default.
• W2912984553 hasPublicationYear "2018" @default.
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