Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912985179> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2912985179 endingPage "567" @default.
- W2912985179 startingPage "545" @default.
- W2912985179 abstract "The various permutations of polymerase chain reaction (PCR) offer the most compelling quantitative expression profile of a sample. A variety of nucleases and approaches have been used to quantify and characterize specific transcripts in greater detail than can be derived from Northern analysis in a non-PCR format. The basic methodology of nuclease protection accommodates denatured double-stranded DNA probes as well as RNA probes. These techniques include primer extension, the nuclear runoff assay, the ribonuclease protection assay and the S1 nuclease protection assay. In the S1 nuclease assay for the quantification of specific RNA species, purified RNA is hybridized in solution with a labeled probe sequence to form thermodynamically stable hybrid molecules. Any RNA or probe molecules that do not participate in the formation of hybrid molecules are digested away by the single-strand-specific nuclease S1, followed by electrophoresis of the intact hybrid molecules. The size and abundance of protected RNAs are then deduced by autoradiography, performed directly from the gel. Whereas in case of RNase protection assay for the quantification of specific RNA species, purified RNA is hybridized in solution with a labeled antisense probe sequence to form thermodynamically stable double-stranded RNA molecules. Any RNA or probe molecules that remain single stranded are digested by an RNase cocktail. Following electrophoresis, the size and abundance of protected RNAs are then deduced by autoradiography, performed directly from the gel. The key advantage that these techniques offer is that they do not require a reverse transcriptase step. Further, because there is no filter-based immobilization on nucleic acid molecules (as in Northern blots), sensitivity is enhanced." @default.
- W2912985179 created "2019-02-21" @default.
- W2912985179 creator A5003710396 @default.
- W2912985179 date "2017-01-01" @default.
- W2912985179 modified "2023-09-26" @default.
- W2912985179 title "Quantification of Specific mRNAs by Nuclease Protection" @default.
- W2912985179 cites W126023894 @default.
- W2912985179 cites W130386017 @default.
- W2912985179 cites W1562167636 @default.
- W2912985179 cites W1607004775 @default.
- W2912985179 cites W1619666977 @default.
- W2912985179 cites W1983403076 @default.
- W2912985179 cites W1996419255 @default.
- W2912985179 cites W2006558753 @default.
- W2912985179 cites W2007918998 @default.
- W2912985179 cites W2021253208 @default.
- W2912985179 cites W2027235289 @default.
- W2912985179 cites W2054073262 @default.
- W2912985179 cites W2072573620 @default.
- W2912985179 cites W2078119356 @default.
- W2912985179 cites W2087800008 @default.
- W2912985179 cites W2091239909 @default.
- W2912985179 cites W2147481206 @default.
- W2912985179 cites W2149611538 @default.
- W2912985179 cites W2157589212 @default.
- W2912985179 cites W2158290831 @default.
- W2912985179 cites W2202673056 @default.
- W2912985179 cites W2210369950 @default.
- W2912985179 cites W2292889715 @default.
- W2912985179 cites W2416571399 @default.
- W2912985179 cites W2743778215 @default.
- W2912985179 cites W998505716 @default.
- W2912985179 doi "https://doi.org/10.1016/b978-0-12-804678-4.00018-x" @default.
- W2912985179 hasPublicationYear "2017" @default.
- W2912985179 type Work @default.
- W2912985179 sameAs 2912985179 @default.
- W2912985179 citedByCount "0" @default.
- W2912985179 crossrefType "book-chapter" @default.
- W2912985179 hasAuthorship W2912985179A5003710396 @default.
- W2912985179 hasConcept C104317684 @default.
- W2912985179 hasConcept C108190873 @default.
- W2912985179 hasConcept C10879258 @default.
- W2912985179 hasConcept C116084860 @default.
- W2912985179 hasConcept C129312508 @default.
- W2912985179 hasConcept C153911025 @default.
- W2912985179 hasConcept C156719811 @default.
- W2912985179 hasConcept C178790620 @default.
- W2912985179 hasConcept C185592680 @default.
- W2912985179 hasConcept C194857396 @default.
- W2912985179 hasConcept C2777271071 @default.
- W2912985179 hasConcept C2777563447 @default.
- W2912985179 hasConcept C2778976237 @default.
- W2912985179 hasConcept C41258723 @default.
- W2912985179 hasConcept C552990157 @default.
- W2912985179 hasConcept C55493867 @default.
- W2912985179 hasConcept C67705224 @default.
- W2912985179 hasConcept C69766542 @default.
- W2912985179 hasConcept C86803240 @default.
- W2912985179 hasConceptScore W2912985179C104317684 @default.
- W2912985179 hasConceptScore W2912985179C108190873 @default.
- W2912985179 hasConceptScore W2912985179C10879258 @default.
- W2912985179 hasConceptScore W2912985179C116084860 @default.
- W2912985179 hasConceptScore W2912985179C129312508 @default.
- W2912985179 hasConceptScore W2912985179C153911025 @default.
- W2912985179 hasConceptScore W2912985179C156719811 @default.
- W2912985179 hasConceptScore W2912985179C178790620 @default.
- W2912985179 hasConceptScore W2912985179C185592680 @default.
- W2912985179 hasConceptScore W2912985179C194857396 @default.
- W2912985179 hasConceptScore W2912985179C2777271071 @default.
- W2912985179 hasConceptScore W2912985179C2777563447 @default.
- W2912985179 hasConceptScore W2912985179C2778976237 @default.
- W2912985179 hasConceptScore W2912985179C41258723 @default.
- W2912985179 hasConceptScore W2912985179C552990157 @default.
- W2912985179 hasConceptScore W2912985179C55493867 @default.
- W2912985179 hasConceptScore W2912985179C67705224 @default.
- W2912985179 hasConceptScore W2912985179C69766542 @default.
- W2912985179 hasConceptScore W2912985179C86803240 @default.
- W2912985179 hasLocation W29129851791 @default.
- W2912985179 hasOpenAccess W2912985179 @default.
- W2912985179 hasPrimaryLocation W29129851791 @default.
- W2912985179 hasRelatedWork W1588442114 @default.
- W2912985179 hasRelatedWork W1967824154 @default.
- W2912985179 hasRelatedWork W1983687016 @default.
- W2912985179 hasRelatedWork W2040575169 @default.
- W2912985179 hasRelatedWork W2072090854 @default.
- W2912985179 hasRelatedWork W2257470766 @default.
- W2912985179 hasRelatedWork W2352699128 @default.
- W2912985179 hasRelatedWork W2396857317 @default.
- W2912985179 hasRelatedWork W2404545519 @default.
- W2912985179 hasRelatedWork W4233738854 @default.
- W2912985179 isParatext "false" @default.
- W2912985179 isRetracted "false" @default.
- W2912985179 magId "2912985179" @default.
- W2912985179 workType "book-chapter" @default.