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• W2912992378 abstract "Hydrogen sulfide (H 2 S) is an important biological messenger, which recently joined nitric oxide (NO) and carbon monoxide (CO) as an endogenously produced gasotransmitter. This discovery resulted in a renewed interest towards H 2 S and, in particular, in its reactivity with biological and bioinorganic targets. However, in the scientific literature studies exploring the reactivity of H 2 S/HS – with non‐heme proteins are rare. In this work, we studied the interaction of HS – with the natural metalloprotein Cu‐azurin and its cobalt variant via UV‐Vis, fluorescence, and EPR spectroscopy, as well as X‐ray diffraction. Interaction of HS – significantly enhances the fluorescence emission of fluorescently labelled copper azurin in buffered solutions and at physiological pH. In contrast, fluorescently labelled cobalt azurin undergoes a fluorescence quenching in the presence of HS – in line with the changes observed in the absorption spectrum of the construct. The results highlight the potential of azurin for implementation in a fast and simple HS – sensor." @default.
• W2912992378 created "2019-02-21" @default.
• W2912992378 creator A5000041874 @default.
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• W2912992378 date "2019-01-21" @default.
• W2912992378 modified "2023-10-16" @default.
• W2912992378 title "Azurin and HS ^– : Towards Implementation of a Sensor for HS ^– Detection" @default.
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• W2912992378 doi "https://doi.org/10.1002/ejic.201801399" @default.
• W2912992378 hasPublicationYear "2019" @default.
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