Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912993280> ?p ?o ?g. }
- W2912993280 endingPage "1678" @default.
- W2912993280 startingPage "1670" @default.
- W2912993280 abstract "Abstract BACKGROUND Microfluidic chips have gained growing attention from the scientific community due to their large surface/interface area and fast mass transfer. However, their application is challenged by the instability of enzymes and time‐consuming catalytic process. Poly ( N‐ isopropylacrylamide) (PNIPAAm) coupled with photopatterning technology was applied to immobilize an enzyme on the microchannel surface to realize the biotransformation in this study. RESULTS The photopattern‐immobilized naringinase on a microchip achieved a yield of 93.63 ± 1.12% for isoquercitrin production within 5 min. The production of unit time per unit enzyme (g g −1 h −1 ) increased 2.1‐fold whereas the reaction time decreased to 1/12 of the time required in a batch reactor. The enzyme was absorbed and desorbed at 40 and 25 °C, respectively, and the release efficiency of immobilized naringinase reached 80.57%, indicating that most of the enzymes were replaced with fresh enzymes to proceed each enzymatic reaction. Six cycles of enzymatic hydrolysis reactions were completed successively, maintaining >60% of relative enzymatic activity. CONCLUSION The results indicated that using the immobilized enzyme on the photopatterned PNIPAAm in the enzymatic hydrolysis of rutin was an efficient and simple way to achieve a high yield of isoquercitrin. Thus, this approach represents a convenient and cost‐saving method to produce fine chemicals using microfluidic biocatalysis. © 2019 Society of Chemical Industry" @default.
- W2912993280 created "2019-02-21" @default.
- W2912993280 creator A5006832647 @default.
- W2912993280 creator A5027947687 @default.
- W2912993280 creator A5069451998 @default.
- W2912993280 creator A5076182575 @default.
- W2912993280 creator A5076856016 @default.
- W2912993280 creator A5086369575 @default.
- W2912993280 creator A5091797690 @default.
- W2912993280 date "2019-03-05" @default.
- W2912993280 modified "2023-10-16" @default.
- W2912993280 title "Enzyme immobilization on photopatterned temperature‐response poly (N‐isopropylacrylamide) for microfluidic biocatalysis" @default.
- W2912993280 cites W1947707933 @default.
- W2912993280 cites W1969304318 @default.
- W2912993280 cites W1979001373 @default.
- W2912993280 cites W1985216885 @default.
- W2912993280 cites W1990151571 @default.
- W2912993280 cites W1998590162 @default.
- W2912993280 cites W1998686419 @default.
- W2912993280 cites W2000505993 @default.
- W2912993280 cites W2004308987 @default.
- W2912993280 cites W2005869545 @default.
- W2912993280 cites W2012247506 @default.
- W2912993280 cites W2045561686 @default.
- W2912993280 cites W2046427711 @default.
- W2912993280 cites W2047244636 @default.
- W2912993280 cites W2048837903 @default.
- W2912993280 cites W2062262742 @default.
- W2912993280 cites W2064745958 @default.
- W2912993280 cites W2068656852 @default.
- W2912993280 cites W2074984370 @default.
- W2912993280 cites W2078411776 @default.
- W2912993280 cites W2079013207 @default.
- W2912993280 cites W2084191509 @default.
- W2912993280 cites W2120726667 @default.
- W2912993280 cites W2134066433 @default.
- W2912993280 cites W2138815275 @default.
- W2912993280 cites W2149242162 @default.
- W2912993280 cites W2154616459 @default.
- W2912993280 cites W2163968375 @default.
- W2912993280 cites W2167848451 @default.
- W2912993280 cites W2237022894 @default.
- W2912993280 cites W2334638239 @default.
- W2912993280 cites W2521739979 @default.
- W2912993280 cites W2550494324 @default.
- W2912993280 cites W2604493652 @default.
- W2912993280 cites W2622562368 @default.
- W2912993280 cites W2715091236 @default.
- W2912993280 cites W2755294234 @default.
- W2912993280 cites W2756209538 @default.
- W2912993280 cites W2769255491 @default.
- W2912993280 cites W2773063136 @default.
- W2912993280 cites W2775448570 @default.
- W2912993280 cites W2791457786 @default.
- W2912993280 doi "https://doi.org/10.1002/jctb.5946" @default.
- W2912993280 hasPublicationYear "2019" @default.
- W2912993280 type Work @default.
- W2912993280 sameAs 2912993280 @default.
- W2912993280 citedByCount "8" @default.
- W2912993280 countsByYear W29129932802019 @default.
- W2912993280 countsByYear W29129932802020 @default.
- W2912993280 countsByYear W29129932802021 @default.
- W2912993280 countsByYear W29129932802022 @default.
- W2912993280 crossrefType "journal-article" @default.
- W2912993280 hasAuthorship W2912993280A5006832647 @default.
- W2912993280 hasAuthorship W2912993280A5027947687 @default.
- W2912993280 hasAuthorship W2912993280A5069451998 @default.
- W2912993280 hasAuthorship W2912993280A5076182575 @default.
- W2912993280 hasAuthorship W2912993280A5076856016 @default.
- W2912993280 hasAuthorship W2912993280A5086369575 @default.
- W2912993280 hasAuthorship W2912993280A5091797690 @default.
- W2912993280 hasConcept C134121241 @default.
- W2912993280 hasConcept C147831808 @default.
- W2912993280 hasConcept C161790260 @default.
- W2912993280 hasConcept C171250308 @default.
- W2912993280 hasConcept C178790620 @default.
- W2912993280 hasConcept C181199279 @default.
- W2912993280 hasConcept C185592680 @default.
- W2912993280 hasConcept C191503008 @default.
- W2912993280 hasConcept C191897082 @default.
- W2912993280 hasConcept C192562407 @default.
- W2912993280 hasConcept C2775963812 @default.
- W2912993280 hasConcept C2778004101 @default.
- W2912993280 hasConcept C2779697368 @default.
- W2912993280 hasConcept C27881333 @default.
- W2912993280 hasConcept C33633552 @default.
- W2912993280 hasConcept C43617362 @default.
- W2912993280 hasConcept C65024703 @default.
- W2912993280 hasConcept C69118441 @default.
- W2912993280 hasConcept C8673954 @default.
- W2912993280 hasConcept C94412978 @default.
- W2912993280 hasConceptScore W2912993280C134121241 @default.
- W2912993280 hasConceptScore W2912993280C147831808 @default.
- W2912993280 hasConceptScore W2912993280C161790260 @default.
- W2912993280 hasConceptScore W2912993280C171250308 @default.
- W2912993280 hasConceptScore W2912993280C178790620 @default.
- W2912993280 hasConceptScore W2912993280C181199279 @default.
- W2912993280 hasConceptScore W2912993280C185592680 @default.