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- W2913014736 abstract "Here, we describe a detailed protocol to set up the αS-PMCA assay using αS synthetic aggregates in buffer and to accurately detect endogenous αS aggregates from human CSF samples. Given the amplificative nature of the technique, minute amounts of misfolded protein aggregates circulating in human bodily fluids can be multiplied and thereafter detected by more conventional methods, such as immune assays or fluorescence. Following these principles, αS-PMCA was standardized for the highly sensitive and specific detection of αS misfolded aggregates in cerebrospinal fluid (CSF) of patients with synucleinopathies." @default.
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- W2913014736 date "2019-01-01" @default.
- W2913014736 modified "2023-10-16" @default.
- W2913014736 title "Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform" @default.
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- W2913014736 doi "https://doi.org/10.1007/978-1-4939-9124-2_4" @default.
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