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• W2913017330 abstract "A biomimetic nanochannel-ionchannel hybrid coupled with electrochemical detector was developed for label-free and ultrasensitive detection of microRNA (miRNA) in cells. Probe single stranded DNA (ssDNA) was first immobilized on the outer surface of the nanochannel-ionchannel hybrid membrane, which can hybridize with the target miRNA in cells. Due to the unique mass transfer property of the hybrid, the DNA-miRNA hybridization kinetics can be sensitively monitored in real-time using the electrochemical technique. More importantly, due to the super small size of the ionchannels, the DNA probe immobilization and hybridization process can be carried out on the outer surface of the ionchannel side, which can effectively avoid the blockage and damage of channels and thus considerably enhance the reproducibility and accuracy of the method. Using this strategy, the miRNA ranging from 0.1 fM to 0.1 μM can be facilely detected with a low detection limit of 15.4 aM, which is much lower than most reported work. The present strategy provides a sensitive and label-free miRNA detection platform, which will be of great significance in biomedical research and clinical diagnosis." @default.
• W2913017330 created "2019-02-21" @default.
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• W2913017330 date "2019-02-13" @default.
• W2913017330 modified "2023-10-14" @default.
• W2913017330 title "Biomimetic Nanochannel-Ionchannel Hybrid for Ultrasensitive and Label-Free Detection of MicroRNA in Cells" @default.
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• W2913017330 doi "https://doi.org/10.1021/acs.analchem.8b05536" @default.
• W2913017330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30758184" @default.
• W2913017330 hasPublicationYear "2019" @default.
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