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- W2913098640 abstract "The fluorescent nitrogen-vacancy (NV) defect in diamond has remarkable photophysical properties, including high photostability and unique sensing properties; as a result, there has been much interest in using nanodiamonds (NDs) for bio-imaging. A facile route for sensing using NDs containing single or multiple NVs is via measuring changes in the NV emission spectra due to chemical (or structural) events on the ND surface, since the NV neutral state (NV0) is 60 nm blue-shifted versus the negative form (NV-). Bio-functionalised NDs have been implemented before in single-molecule imaging; however, the effect of functionalisation on ND's photophysics has been poorly studied due to a lack of high-throughput characterisation methods. Such effects are important to understand and control, since biomolecules may affect the NV charge, thus causing spectral shifts and possibly blinking. Here, we report a high-throughput analysis of NDs using wide-field epifluorescence dual-colour imaging. We have measured the proportion of single-NVs in fluorescent NDs of different sizes (10 - 100 nm) by analysing their intensity distribution, and found that it was in the 50-80% range. Using the ratio of green and red intensities, we have directly observed the charge conversion of single NVs, and, for the first time, we are able to measure the lifetimes and transition rates between charge states in NDs. We also show that the NV's charge changes in a pH-dependent fashion in uncoated NDs (<20 nm diameter), opening the possibility of using NDs for intracellular pH sensing and mapping. Finally, we investigate the photophysics of NDs conjugated to double-stranded DNA and to proteins involved in bacterial transcription with a view to developing assays for transcriptional studies. Our results provide us with a new insight into the ND photophysics and their nanoscale sensing capabilities." @default.
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- W2913098640 date "2019-02-01" @default.
- W2913098640 modified "2023-10-16" @default.
- W2913098640 title "Single Nitrogen-Vacancy Imaging in Nanodiamonds for Multimodal Sensing" @default.
- W2913098640 doi "https://doi.org/10.1016/j.bpj.2018.11.966" @default.
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