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• W2913149034 abstract "BackgroundStem cell-derived by conditioned medium has potential as regenerative agent of skin cells because it contains many growth factors, cytokines and other regenerative biomolecules. Conditioned medium (CM) from human adipose tissue-derived MSCs (CM-hATMSCs) also has been known enriched growth factors that play an important role in epithelial wound repair, reduce wrinkles, and enhance wound healing.ObjectiveThis study was performed to evaluate growth factors in CM such as Transforming Growth Factor (TGF)-β1, TGF-β2, Vascular Endothelial Growth Factor (VEGF)-2, Vascular Cell Adhesion Molecule (VCAM) 1, Epidermal Growth Factor (EGF), Fibroblast Growth Factor (FGF) in CM-hATMSCs treated with Fresh Frozen Plasma (FFP) and non-FFP at various passages.MethodsThis study used ELISA method to measure the growth factors in CM-hATMSCs.ResultsFGF was the highest modulator among FFP and non-FFP-treated cells at many passages compared to the others growth factor. Both FFP and non-FFP-treated cells showed significant difference (P < 0.05) in some growth factors. However, there was no significant differences in TGF-β1 at passage 3,7,11 and 15 in FFP-treated cells and non-FFP-treated cells.ConclusionIn summary, the highest concentration level found in CM-hATMSCs is FGF both in FFP and non-FFP-treated cells. FGF as growth factor composition in CM-hATMSCs has potential as wound healing and regeneration of cell and it can be used in anti-aging products. Stem cell-derived by conditioned medium has potential as regenerative agent of skin cells because it contains many growth factors, cytokines and other regenerative biomolecules. Conditioned medium (CM) from human adipose tissue-derived MSCs (CM-hATMSCs) also has been known enriched growth factors that play an important role in epithelial wound repair, reduce wrinkles, and enhance wound healing. This study was performed to evaluate growth factors in CM such as Transforming Growth Factor (TGF)-β1, TGF-β2, Vascular Endothelial Growth Factor (VEGF)-2, Vascular Cell Adhesion Molecule (VCAM) 1, Epidermal Growth Factor (EGF), Fibroblast Growth Factor (FGF) in CM-hATMSCs treated with Fresh Frozen Plasma (FFP) and non-FFP at various passages. This study used ELISA method to measure the growth factors in CM-hATMSCs. FGF was the highest modulator among FFP and non-FFP-treated cells at many passages compared to the others growth factor. Both FFP and non-FFP-treated cells showed significant difference (P < 0.05) in some growth factors. However, there was no significant differences in TGF-β1 at passage 3,7,11 and 15 in FFP-treated cells and non-FFP-treated cells. In summary, the highest concentration level found in CM-hATMSCs is FGF both in FFP and non-FFP-treated cells. FGF as growth factor composition in CM-hATMSCs has potential as wound healing and regeneration of cell and it can be used in anti-aging products." @default.
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• W2913149034 date "2019-04-01" @default.
• W2913149034 modified "2023-09-24" @default.
• W2913149034 title "Growth factors profile in conditioned medium human adipose tissue-derived mesenchymal stem cells (CM-hATMSCs)" @default.
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• W2913149034 doi "https://doi.org/10.1016/j.yclnex.2019.01.002" @default.
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