Matches in SemOpenAlex for { <https://semopenalex.org/work/W2913152203> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2913152203 endingPage "471a" @default.
- W2913152203 startingPage "471a" @default.
- W2913152203 abstract "Neuroglobin (Ngb) is a new member of hexa-coordinate vertebrate Heme-globin with several proposed functions such as ROS/RNS scavenging, and inhibition of hypoxia-induced apoptosis. Nonetheless, the molecular mechanism for most of its functions is still not clear. Recent studies have shown a link between the structure and function of the protein, for example, the CD loop in Ngb is a potential binding site for the G-alpha subunit and the distal histidine affects the thermodynamic profile for gaseous ligands association. In order to characterize the structure function relationship in Ngb, we have introduced two mutants, the E53Q in the CD loop region and H64Q in the distal pocket. UV-vis and fluorescence spectroscopy together with time of flight-mass spectrometry have been used to determine how the mutations affect the Ngb stability. Our data indicate a distinct stability profile of the two mutants in both liquid phase and gas phase. In the gas phase, Ngb WT is more stable in neutral pH whereas stability of Ngb mutants was pH independent. However, both mutants, especially E53Q, are significantly less stable in the gas phase than the wild type. In the liquid phase, distal histidine replacement leads to a decrease in overall protein stability whereas the stability of E53Q mutant is comparable to that of WT. Nevertheless, the replacement of glutamine residue increases the protein solubility during pH induced unfolding studies. Overall, our data provide information on the impact of the CD loop and the distal histidine on protein stability in both gas phase and liquid phase." @default.
- W2913152203 created "2019-02-21" @default.
- W2913152203 creator A5025456484 @default.
- W2913152203 creator A5047799551 @default.
- W2913152203 creator A5053947295 @default.
- W2913152203 creator A5065406786 @default.
- W2913152203 creator A5073769483 @default.
- W2913152203 date "2019-02-01" @default.
- W2913152203 modified "2023-09-26" @default.
- W2913152203 title "Mutation of Residues in CD Loop and Distal Pocket Impact Protein Stability of Human Neuroglobin" @default.
- W2913152203 doi "https://doi.org/10.1016/j.bpj.2018.11.2545" @default.
- W2913152203 hasPublicationYear "2019" @default.
- W2913152203 type Work @default.
- W2913152203 sameAs 2913152203 @default.
- W2913152203 citedByCount "0" @default.
- W2913152203 crossrefType "journal-article" @default.
- W2913152203 hasAuthorship W2913152203A5025456484 @default.
- W2913152203 hasAuthorship W2913152203A5047799551 @default.
- W2913152203 hasAuthorship W2913152203A5053947295 @default.
- W2913152203 hasAuthorship W2913152203A5065406786 @default.
- W2913152203 hasAuthorship W2913152203A5073769483 @default.
- W2913152203 hasBestOaLocation W29131522031 @default.
- W2913152203 hasConcept C104292427 @default.
- W2913152203 hasConcept C104317684 @default.
- W2913152203 hasConcept C12554922 @default.
- W2913152203 hasConcept C125996951 @default.
- W2913152203 hasConcept C143065580 @default.
- W2913152203 hasConcept C181199279 @default.
- W2913152203 hasConcept C185592680 @default.
- W2913152203 hasConcept C2776217839 @default.
- W2913152203 hasConcept C2776318716 @default.
- W2913152203 hasConcept C2778460671 @default.
- W2913152203 hasConcept C2778917026 @default.
- W2913152203 hasConcept C515207424 @default.
- W2913152203 hasConcept C55493867 @default.
- W2913152203 hasConcept C86803240 @default.
- W2913152203 hasConceptScore W2913152203C104292427 @default.
- W2913152203 hasConceptScore W2913152203C104317684 @default.
- W2913152203 hasConceptScore W2913152203C12554922 @default.
- W2913152203 hasConceptScore W2913152203C125996951 @default.
- W2913152203 hasConceptScore W2913152203C143065580 @default.
- W2913152203 hasConceptScore W2913152203C181199279 @default.
- W2913152203 hasConceptScore W2913152203C185592680 @default.
- W2913152203 hasConceptScore W2913152203C2776217839 @default.
- W2913152203 hasConceptScore W2913152203C2776318716 @default.
- W2913152203 hasConceptScore W2913152203C2778460671 @default.
- W2913152203 hasConceptScore W2913152203C2778917026 @default.
- W2913152203 hasConceptScore W2913152203C515207424 @default.
- W2913152203 hasConceptScore W2913152203C55493867 @default.
- W2913152203 hasConceptScore W2913152203C86803240 @default.
- W2913152203 hasIssue "3" @default.
- W2913152203 hasLocation W29131522031 @default.
- W2913152203 hasOpenAccess W2913152203 @default.
- W2913152203 hasPrimaryLocation W29131522031 @default.
- W2913152203 hasRelatedWork W1975030797 @default.
- W2913152203 hasRelatedWork W1990357304 @default.
- W2913152203 hasRelatedWork W2044372603 @default.
- W2913152203 hasRelatedWork W2054976302 @default.
- W2913152203 hasRelatedWork W2058284268 @default.
- W2913152203 hasRelatedWork W2065350586 @default.
- W2913152203 hasRelatedWork W2075841103 @default.
- W2913152203 hasRelatedWork W2089993404 @default.
- W2913152203 hasRelatedWork W2093921132 @default.
- W2913152203 hasRelatedWork W2156688576 @default.
- W2913152203 hasVolume "116" @default.
- W2913152203 isParatext "false" @default.
- W2913152203 isRetracted "false" @default.
- W2913152203 magId "2913152203" @default.
- W2913152203 workType "article" @default.