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• W2913172812 abstract "The aim of this research project was the prove of gypsy-like sequences in the genome of the cat flea. Therefore nested PCR was practiced with two degenerated primers from the study of VAZQUEZ-MANRIQUE et al. (2000). Before trying to amplify gypsy-like sequences in the genome of C. felis, the gypsyvirus was proved in the genome of Drosophila melanogaster, in order to guarantee the functionning of this very sensitive PCR-method. PCR was done, using genomic DNA of the cat flea as template. A gypsy-positive french D. melanogaster strain was used as positive control all the time. The hybridization of the cat flea-PCR-products was carried out with a french gypsy-positive D. melanogaster probe. It was possible to prove a 410 bp  gypsy-like sequence in the genome of C. felis (genomic DNA of culture- and wildtypepopulations were pooled), which was derived from the end of the pol-gene-region and the beginning of the env-gene of D. melanogaster. This amplified sequence was characteristic of an imperfect sequence because of three deletions, of which the two biggest (26 bp and 146 bp in length) were observed in the env-gene-region. At the 410 bp gypsy-like sequence of the cat flea one forward and two reverse primers were chosen for PCR with D. melanogaster and C. felis. With this experiment the “flea-specifity” of the cat flea-sequence could be proved and contaminations could be shut out simultaneously. The PCR-product, which was received from the cat flea in this experiment, was used as a  flea-specific gypsy-like probe for hybridization and therefore served as prove of “gypsy-specifity”. In four southern blots the genomic DNA of the fruit fly and the cat flea were hybridizised on the one hand with the gypsy-positive french D. melanogaster-probe and on the other hand with the gypsy-like C. felis-probe. With this experiment the “flea-specifity” and “gypsy-specifity” of the 410 bp sequence in the genome of the cat flea could be proved.  1)     1)     So there exist gypsy-like sequences in the genome of the cat flea. 2)     2)     The amplified 410 bp sequence in the genome of the cat flea is characteristic of an imperfect sequence because of the three deletions. Because of the stop codons in this sequence, the env-gene is not able to translate functionning envelope proteins. 3)     3)     Retrotransposons are wide-spread in the genome of many organisms and the phylogenetic degree of relationship between the Diptera and Siphonaptera seems quite closely. In consideration of region-specific differences relative to the occurence of this insect Errantivirus, the presence of perfect gypsy-sequences, which could be able to encode for functional proteins, in addition to the imperfect one in the genome of C. felis seems possible. 4)     4)     C. felis is able to transmit viruses, so that this parasite could play on principle a vector role in the potential transmission of gypsyviruses provided that there are viral particles in its salivary gland. 5)     5)     This could possibly mean a progress for the researches of many diseases of the hosts of  C. felis, which are characterized by a high mutation rate." @default.
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• W2913172812 date "2002-01-01" @default.
• W2913172812 modified "2023-09-27" @default.
• W2913172812 title "Nachweis gypsy-ähnlicher Sequenzen im Genom des Katzenflohs, Ctenocephalides felis felis (Bouché 1835)" @default.
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