FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2913231373> ?p ?o ?g. }

• W2913231373 abstract "Neospora caninum is an obligate intracellular apicomplexan parasite, the etiologic agent of neosporosis, and a major cause of reproductive loss in cattle. There is still a lack of effective prevention and treatment measures. The 14-3-3 protein is a widely expressed acidic protein that spontaneously forms dimers within apicomplexan parasites. This protein has been isolated and sequenced in many parasites; however, there are few reports about the N. caninum 14-3-3 protein. Here, we successfully expressed and purified a recombinant fusion protein of Nc14-3-3 (rNc14-3-3) and prepared a polyclonal antibody. Immunofluorescence and immunogold electron microscopy studies of tachyzoites or N. caninum-infected cells suggested that 14-3-3 was localized in the cytosol and the membrane. Western blotting analysis indicated that rNc14-3-3 could be recognized by N. caninum-infected mouse sera, suggesting that 14-3-3 may be an infection-associated antigen that is involved in the host immune response. We demonstrated that rNc14-3-3 induced cytokine expression by activating the MAPK and AKT signaling pathways, and inhibitors of p38, ERK, JNK and AKT could significantly decrease the production of IL-6, IL-12p40 and TNF-α. In addition, phosphorylated nuclear factor-κB (NF-κB/p65) was observed in wild-type peritoneal macrophages treated with rNc14-3-3, and the protein level of NF-κB/p65 was reduced in the cytoplasm but increased correspondingly in the nucleus after 2 h of treatment. These results were also observed in deficient in TLR2-/- peritoneal macrophages. Taken together, our results indicated that the N. caninum 14-3-3 protein can induce effective immune responses and stimulate cytokine expression by activating the MAPK, AKT and NF-κB signaling pathways but did not dependent TLR2, suggesting that Nc14-3-3 is a novel vaccine candidate against neosporosis." @default.
• W2913231373 created "2019-02-21" @default.
• W2913231373 creator A5005287838 @default.
• W2913231373 creator A5009318195 @default.
• W2913231373 creator A5027497045 @default.
• W2913231373 creator A5034092020 @default.
• W2913231373 creator A5038712738 @default.
• W2913231373 creator A5046380471 @default.
• W2913231373 creator A5048803670 @default.
• W2913231373 creator A5052856299 @default.
• W2913231373 creator A5056101043 @default.
• W2913231373 creator A5084641325 @default.
• W2913231373 creator A5091077688 @default.
• W2913231373 date "2019-01-25" @default.
• W2913231373 modified "2023-09-24" @default.
• W2913231373 title "14-3-3 Protein of Neospora caninum Modulates Host Cell Innate Immunity Through the Activation of MAPK and NF-κB Pathways" @default.
• W2913231373 cites W1629604081 @default.
• W2913231373 cites W1967313871 @default.
• W2913231373 cites W1972008234 @default.
• W2913231373 cites W1981577958 @default.
• W2913231373 cites W1984621921 @default.
• W2913231373 cites W1984944574 @default.
• W2913231373 cites W1986221548 @default.
• W2913231373 cites W1993216157 @default.
• W2913231373 cites W1994958449 @default.
• W2913231373 cites W1997940063 @default.
• W2913231373 cites W2001213946 @default.
• W2913231373 cites W2019082340 @default.
• W2913231373 cites W2026234221 @default.
• W2913231373 cites W2043663764 @default.
• W2913231373 cites W2044400952 @default.
• W2913231373 cites W2048168473 @default.
• W2913231373 cites W2051851091 @default.
• W2913231373 cites W2051881983 @default.
• W2913231373 cites W2055694420 @default.
• W2913231373 cites W2057064774 @default.
• W2913231373 cites W2073132533 @default.
• W2913231373 cites W2091366956 @default.
• W2913231373 cites W2100577789 @default.
• W2913231373 cites W2100868544 @default.
• W2913231373 cites W2101249834 @default.
• W2913231373 cites W2109370637 @default.
• W2913231373 cites W2109491705 @default.
• W2913231373 cites W2114671362 @default.
• W2913231373 cites W2122202829 @default.
• W2913231373 cites W2122772601 @default.
• W2913231373 cites W2126882695 @default.
• W2913231373 cites W2138934663 @default.
• W2913231373 cites W2156016194 @default.
• W2913231373 cites W2165622290 @default.
• W2913231373 cites W2189852070 @default.
• W2913231373 cites W2195325770 @default.
• W2913231373 cites W2307984791 @default.
• W2913231373 cites W2471325552 @default.
• W2913231373 cites W2519266528 @default.
• W2913231373 cites W2559476314 @default.
• W2913231373 cites W2590614589 @default.
• W2913231373 cites W2598734543 @default.
• W2913231373 cites W2606513998 @default.
• W2913231373 cites W2617157549 @default.
• W2913231373 cites W2739422012 @default.
• W2913231373 cites W2754696525 @default.
• W2913231373 cites W2761815088 @default.
• W2913231373 cites W2825387050 @default.
• W2913231373 doi "https://doi.org/10.3389/fmicb.2019.00037" @default.
• W2913231373 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6355710" @default.
• W2913231373 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30740096" @default.
• W2913231373 hasPublicationYear "2019" @default.
• W2913231373 type Work @default.
• W2913231373 sameAs 2913231373 @default.
• W2913231373 citedByCount "10" @default.
• W2913231373 countsByYear W29132313732020 @default.
• W2913231373 countsByYear W29132313732021 @default.
• W2913231373 countsByYear W29132313732022 @default.
• W2913231373 countsByYear W29132313732023 @default.
• W2913231373 crossrefType "journal-article" @default.
• W2913231373 hasAuthorship W2913231373A5005287838 @default.
• W2913231373 hasAuthorship W2913231373A5009318195 @default.
• W2913231373 hasAuthorship W2913231373A5027497045 @default.
• W2913231373 hasAuthorship W2913231373A5034092020 @default.
• W2913231373 hasAuthorship W2913231373A5038712738 @default.
• W2913231373 hasAuthorship W2913231373A5046380471 @default.
• W2913231373 hasAuthorship W2913231373A5048803670 @default.
• W2913231373 hasAuthorship W2913231373A5052856299 @default.
• W2913231373 hasAuthorship W2913231373A5056101043 @default.
• W2913231373 hasAuthorship W2913231373A5084641325 @default.
• W2913231373 hasAuthorship W2913231373A5091077688 @default.
• W2913231373 hasBestOaLocation W29132313731 @default.
• W2913231373 hasConcept C136449434 @default.
• W2913231373 hasConcept C153911025 @default.
• W2913231373 hasConcept C159654299 @default.
• W2913231373 hasConcept C203014093 @default.
• W2913231373 hasConcept C2778128430 @default.
• W2913231373 hasConcept C2781154746 @default.
• W2913231373 hasConcept C2781236682 @default.
• W2913231373 hasConcept C86803240 @default.
• W2913231373 hasConcept C8891405 @default.
• W2913231373 hasConcept C89423630 @default.
• W2913231373 hasConcept C95444343 @default.
• W2913231373 hasConceptScore W2913231373C136449434 @default.

• next