Matches in SemOpenAlex for { <https://semopenalex.org/work/W2913305736> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2913305736 endingPage "1990" @default.
- W2913305736 startingPage "1984" @default.
- W2913305736 abstract "Capripox viruses of small ruminants, namely goatpox virus (GTPV) and sheep pox virus (SPPV) are responsible for important contagious diseases that are enzootic to the Indian sub-continent, Africa and the Middle East. In the present study, recombinant F13L and P32 proteins of GTPV were expressed in prokaryotic system, purified and confirmed in Western blot in order to evaluate their diagnostic potential. Full length F13L (1M-L370aa) and truncated P32 (20V-S270aa) genes of GTPV-Uttarkashi strain were cloned into pET-33b(+) vector, over-expressed in prokaryotic system and purified as histidine-tagged protein using Ni-NTA affinity chromatography under denaturing conditions and passive elution method, respectively. The recombinantF13Land P32 proteins lacked fusion tag from vector except histidine tag for purification as analyzed by SDS-PAGE. Expression was confirmed with Western blot using anti-GTPV serum. The purified recombinant F13L and P32 proteins can be used potential diagnostic antigen/s either individually or in combination for sero-diagnosis of capripoxvirus infections." @default.
- W2913305736 created "2019-02-21" @default.
- W2913305736 creator A5004559347 @default.
- W2913305736 creator A5008102640 @default.
- W2913305736 creator A5015245845 @default.
- W2913305736 creator A5053311592 @default.
- W2913305736 creator A5053547541 @default.
- W2913305736 creator A5069080399 @default.
- W2913305736 date "2019-01-10" @default.
- W2913305736 modified "2023-10-16" @default.
- W2913305736 title "Expression and Purification of Recombinant Immunogenic Proteins of Goat Poxvirus in Prokaryotic System" @default.
- W2913305736 cites W1515844392 @default.
- W2913305736 cites W2056191056 @default.
- W2913305736 cites W2067147275 @default.
- W2913305736 cites W2085810343 @default.
- W2913305736 cites W2100170686 @default.
- W2913305736 cites W2129683254 @default.
- W2913305736 cites W2155402817 @default.
- W2913305736 cites W2162041362 @default.
- W2913305736 cites W2227722006 @default.
- W2913305736 cites W2238899646 @default.
- W2913305736 cites W2562048827 @default.
- W2913305736 cites W2771055673 @default.
- W2913305736 cites W2888368222 @default.
- W2913305736 doi "https://doi.org/10.20546/ijcmas.2019.801.208" @default.
- W2913305736 hasPublicationYear "2019" @default.
- W2913305736 type Work @default.
- W2913305736 sameAs 2913305736 @default.
- W2913305736 citedByCount "2" @default.
- W2913305736 countsByYear W29133057362020 @default.
- W2913305736 countsByYear W29133057362021 @default.
- W2913305736 crossrefType "journal-article" @default.
- W2913305736 hasAuthorship W2913305736A5004559347 @default.
- W2913305736 hasAuthorship W2913305736A5008102640 @default.
- W2913305736 hasAuthorship W2913305736A5015245845 @default.
- W2913305736 hasAuthorship W2913305736A5053311592 @default.
- W2913305736 hasAuthorship W2913305736A5053547541 @default.
- W2913305736 hasAuthorship W2913305736A5069080399 @default.
- W2913305736 hasBestOaLocation W29133057361 @default.
- W2913305736 hasConcept C104317684 @default.
- W2913305736 hasConcept C123894998 @default.
- W2913305736 hasConcept C153911025 @default.
- W2913305736 hasConcept C159047783 @default.
- W2913305736 hasConcept C179247698 @default.
- W2913305736 hasConcept C181199279 @default.
- W2913305736 hasConcept C2776415932 @default.
- W2913305736 hasConcept C40767141 @default.
- W2913305736 hasConcept C55493867 @default.
- W2913305736 hasConcept C86803240 @default.
- W2913305736 hasConceptScore W2913305736C104317684 @default.
- W2913305736 hasConceptScore W2913305736C123894998 @default.
- W2913305736 hasConceptScore W2913305736C153911025 @default.
- W2913305736 hasConceptScore W2913305736C159047783 @default.
- W2913305736 hasConceptScore W2913305736C179247698 @default.
- W2913305736 hasConceptScore W2913305736C181199279 @default.
- W2913305736 hasConceptScore W2913305736C2776415932 @default.
- W2913305736 hasConceptScore W2913305736C40767141 @default.
- W2913305736 hasConceptScore W2913305736C55493867 @default.
- W2913305736 hasConceptScore W2913305736C86803240 @default.
- W2913305736 hasIssue "01" @default.
- W2913305736 hasLocation W29133057361 @default.
- W2913305736 hasOpenAccess W2913305736 @default.
- W2913305736 hasPrimaryLocation W29133057361 @default.
- W2913305736 hasRelatedWork W2360222450 @default.
- W2913305736 hasRelatedWork W2362612214 @default.
- W2913305736 hasRelatedWork W2371993564 @default.
- W2913305736 hasRelatedWork W2373329635 @default.
- W2913305736 hasRelatedWork W2377360890 @default.
- W2913305736 hasRelatedWork W2381845779 @default.
- W2913305736 hasRelatedWork W2381958483 @default.
- W2913305736 hasRelatedWork W2431377509 @default.
- W2913305736 hasRelatedWork W276272931 @default.
- W2913305736 hasRelatedWork W3149679323 @default.
- W2913305736 hasVolume "8" @default.
- W2913305736 isParatext "false" @default.
- W2913305736 isRetracted "false" @default.
- W2913305736 magId "2913305736" @default.
- W2913305736 workType "article" @default.