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• W2913355559 abstract "HomePlant DiseaseVol. 103, No. 6Bacterial Blight on Millet Caused by Burkholderia cepacia in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseBacterial Blight on Millet Caused by Burkholderia cepacia in BrazilC. S. Conceição, A. D. B. Baia, P. H. R. Silva, A. M. F. Silva, A. J. G. Moraes, E. B. Souza, J. E. A. Beserra Junior, M. P. Melo, and M. A. S. GamaC. S. ConceiçãoDepartamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil; Search for more papers by this author, A. D. B. BaiaDepartamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil; Search for more papers by this author, P. H. R. SilvaDepartamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil; Search for more papers by this author, A. M. F. SilvaDepartamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil; Search for more papers by this author, A. J. G. MoraesDepartamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil; Search for more papers by this author, E. B. SouzaDepartamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil; Search for more papers by this author, J. E. A. Beserra JuniorDepartamento de Fitotecnia, Universidade Federal do Piauí, Teresina, 64049-550, Piauí, Brazil; and Search for more papers by this author, M. P. MeloUniversity Federal do Oeste do Pará, Campus Juruti, 68170-000, Juruti, PA, BrazilSearch for more papers by this author, and M. A. S. Gama†Corresponding author: M. A. S. Gama; E-mail Address: mas.gama@yahoo.com.brhttp://orcid.org/0000-0001-6153-1700Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil; Search for more papers by this authorAffiliationsAuthors and Affiliations C. S. Conceição1 A. D. B. Baia1 P. H. R. Silva1 A. M. F. Silva1 A. J. G. Moraes1 E. B. Souza2 J. E. A. Beserra Junior3 M. P. Melo4 M. A. S. Gama1 † 1Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil; 2Departamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil; 3Departamento de Fitotecnia, Universidade Federal do Piauí, Teresina, 64049-550, Piauí, Brazil; and 4University Federal do Oeste do Pará, Campus Juruti, 68170-000, Juruti, PA, Brazil Published Online:21 Mar 2019https://doi.org/10.1094/PDIS-10-18-1894-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Millet (Pennisetum glaucum) is a cereal crop of high importance in some regions of the world, and in Brazil the flour is mainly used for the preparation of cakes, biscuits, and porridge. In March 2015, we observed symptoms of yellowing, chlorosis, and blight on millet leaves of cultivar ADR-300 (about 30% incidence) in a plantation located in the municipality of Baixa Grande do Ribeiro (07°51′32″S, 45°12′56″W), Piauí state, Brazil. Samples were collected from a millet field, and five bacterial strains (CCRMBC01 to CCRMBC05) were isolated in trypan blue tetracycline medium (Hagedorn et al. 1987). Pathogenicity tests were performed on 35-day-old millet plants of cultivar BRS 1501, and inoculations were carried out by foliar spraying of bacterial suspension (108 CFU/ml). The negative control consisted of leaves treated with sterile distilled water (SDW). We used three replicates each with three plants. Inoculated leaves showed symptoms of yellowing, chlorosis, and blight 5 days after inoculation, whereas leaves treated with SDW remained symptomless. Seven days after inoculation we fulfilled Koch’s postulates by reisolating the pathogen. BOX-A1R-based BOX sequence polymerase chain reaction (BOX-PCR) analyses were performed according to the method of Louws et al. (1994) to confirm the identity of the strains that were reisolated from Koch’s postulates. The five strains showed the same BOX-PCR profile and were considered clones. For this reason, only the strain CCRMBC01 was used in the biochemical and phylogenetic analyses. The Biolog Gen III system was used for the biochemical identification, and the strain CCRMBC01 was identified as Burkholderia cepacia at a 67% similarity level. The molecular identification was performed by sequencing a 1,500-bp fragment of the 16S rRNA region (Weisburg et al. 1991), a 376-bp fragment of the recA gene (Ginther et al. 2015), and a 454-bp fragment of the gyrB gene (Spilker et al. 2009). Using the BLASTn tool, we observed that sequences of the 16S rRNA region (GenBank MH622193), recA (GenBank MH631060), and gyrB (GenBank MH631060) genes of the strain CCRMBC01 showed 99, 99, and 98% of identity, respectively, with sequences from the type strain of B. cepacia (LMG 1222T; GenBank AF416381, FJ670559, and AY996867). In the 16S rRNA region maximum likelihood phylogenetic tree, the strain CCRMBC01 clustered with several strains of the B. cepacia complex (BCC), including the type strain of B. cepacia (LMG 1222T, AF416381). Phylogenetic analysis of concatenated sequences of the recA and gyrB genes based on maximum likelihood, along with sequences from all the types or representative strains of BCC (https://pubmlst.org/bcc/) showed that the strain CCRMBC01 clustered with the LMG 1222T strain of B. cepacia, with 99% bootstrap support. From our information, this is the first report of B. cepacia causing bacterial blight in millet in Brazil and in the world. In addition, this report sheds light on the host range for B. cepacia, which is a bacterium that has been neglected as a plant pathogen.The author(s) declare no conflict of interest.References:Ginther, J. L., et al. 2015. PLoS Negl. Trop. Dis. 9:e0003892. https://doi.org/10.1371/journal.pntd.0003892 Crossref, ISI, Google ScholarHagedorn, C., et al. 1987. Appl. Environ. Microbiol. 59:2265. Crossref, ISI, Google ScholarLouws, E. J., et al. 1994. Appl. Environ. Microbiol. 60:2286. Crossref, ISI, Google ScholarSpilker, T., et al. 2009. J. Clin. Microbiol. 47:2607. https://doi.org/10.1128/JCM.00770-09 Crossref, ISI, Google ScholarWeisburg, W. G., et al. 1991. J. Bacteriol. 173:697. https://doi.org/10.1128/jb.173.2.697-703.1991 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 103, No. 6 June 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionGreen mottle mosaic and leaf deformation symptoms on watermelon (Sui, Li, Shamimuzzaman, Wu, and Ling). Photo credit: K.-S. Ling. Postharvest rot on cucumber caused by Ceratocystis fimbriata (Li, Xu, Zhang, Song, Xie, Sun, and Huang). Photo credit: H. Song. Metrics Article History Issue Date: 6 Jun 2019Published: 21 Mar 2019First Look: 23 Jan 2019Accepted: 21 Jan 2019 Page: 1408 Information© 2019 The American Phytopathological SocietyThe author(s) declare no conflict of interest.Cited bySecondary invader bacteria associated with the red pest weevil infestation in date palm trees18 August 2021 | Agronomy Journal, Vol. 113, No. 5" @default.
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