Matches in SemOpenAlex for { <https://semopenalex.org/work/W2913372637> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2913372637 endingPage "600" @default.
- W2913372637 startingPage "596" @default.
- W2913372637 abstract "A typical molecular cloning procedure requires Sanger sequencing for sequence validation, which is cost-prohibitive and labor-intensive for large-scale clone analysis in genotype–phenotype studies. Here we present the cost-effective clone analysis platform TnClone, which uses next-generation sequencing based on Tn5 tagmentation to rapidly analyze a large number of clones from cell lysates. This method bypasses the extensive plasmid purification step. We also developed a user-friendly graphical user interface and provided general guidelines for conducting validation experiments. We tested our program with 1023 plasmids (222 from cell lysates and 801 from purified clones) and achieved 92% and 99.3% sensitivity with cell lysates and purified DNA, respectively. Our platform provides rapid turnaround with minimal hands-on time for secondary evaluation, as next-generation sequencing technology continues to evolve." @default.
- W2913372637 created "2019-02-21" @default.
- W2913372637 creator A5001631124 @default.
- W2913372637 creator A5015197489 @default.
- W2913372637 creator A5047403943 @default.
- W2913372637 creator A5090023484 @default.
- W2913372637 creator A5091289899 @default.
- W2913372637 date "2019-02-06" @default.
- W2913372637 modified "2023-10-14" @default.
- W2913372637 title "Facilitated Large-Scale Sequence Validation Platform Using Tn5-Tagmented Cell Lysates" @default.
- W2913372637 cites W2026863186 @default.
- W2913372637 cites W2120902911 @default.
- W2913372637 cites W2129933858 @default.
- W2913372637 cites W2138270253 @default.
- W2913372637 cites W2157539385 @default.
- W2913372637 cites W2318806263 @default.
- W2913372637 doi "https://doi.org/10.1021/acssynbio.8b00482" @default.
- W2913372637 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30726053" @default.
- W2913372637 hasPublicationYear "2019" @default.
- W2913372637 type Work @default.
- W2913372637 sameAs 2913372637 @default.
- W2913372637 citedByCount "7" @default.
- W2913372637 countsByYear W29133726372020 @default.
- W2913372637 countsByYear W29133726372021 @default.
- W2913372637 countsByYear W29133726372022 @default.
- W2913372637 countsByYear W29133726372023 @default.
- W2913372637 crossrefType "journal-article" @default.
- W2913372637 hasAuthorship W2913372637A5001631124 @default.
- W2913372637 hasAuthorship W2913372637A5015197489 @default.
- W2913372637 hasAuthorship W2913372637A5047403943 @default.
- W2913372637 hasAuthorship W2913372637A5090023484 @default.
- W2913372637 hasAuthorship W2913372637A5091289899 @default.
- W2913372637 hasBestOaLocation W29133726372 @default.
- W2913372637 hasConcept C111919701 @default.
- W2913372637 hasConcept C121050878 @default.
- W2913372637 hasConcept C176553487 @default.
- W2913372637 hasConcept C199360897 @default.
- W2913372637 hasConcept C22744801 @default.
- W2913372637 hasConcept C41008148 @default.
- W2913372637 hasConcept C51679486 @default.
- W2913372637 hasConcept C54355233 @default.
- W2913372637 hasConcept C552990157 @default.
- W2913372637 hasConcept C60644358 @default.
- W2913372637 hasConcept C61053724 @default.
- W2913372637 hasConcept C70721500 @default.
- W2913372637 hasConcept C76818968 @default.
- W2913372637 hasConcept C81089528 @default.
- W2913372637 hasConcept C86803240 @default.
- W2913372637 hasConceptScore W2913372637C111919701 @default.
- W2913372637 hasConceptScore W2913372637C121050878 @default.
- W2913372637 hasConceptScore W2913372637C176553487 @default.
- W2913372637 hasConceptScore W2913372637C199360897 @default.
- W2913372637 hasConceptScore W2913372637C22744801 @default.
- W2913372637 hasConceptScore W2913372637C41008148 @default.
- W2913372637 hasConceptScore W2913372637C51679486 @default.
- W2913372637 hasConceptScore W2913372637C54355233 @default.
- W2913372637 hasConceptScore W2913372637C552990157 @default.
- W2913372637 hasConceptScore W2913372637C60644358 @default.
- W2913372637 hasConceptScore W2913372637C61053724 @default.
- W2913372637 hasConceptScore W2913372637C70721500 @default.
- W2913372637 hasConceptScore W2913372637C76818968 @default.
- W2913372637 hasConceptScore W2913372637C81089528 @default.
- W2913372637 hasConceptScore W2913372637C86803240 @default.
- W2913372637 hasFunder F4320322034 @default.
- W2913372637 hasFunder F4320322120 @default.
- W2913372637 hasIssue "3" @default.
- W2913372637 hasLocation W29133726371 @default.
- W2913372637 hasLocation W29133726372 @default.
- W2913372637 hasLocation W29133726373 @default.
- W2913372637 hasOpenAccess W2913372637 @default.
- W2913372637 hasPrimaryLocation W29133726371 @default.
- W2913372637 hasRelatedWork W1983392759 @default.
- W2913372637 hasRelatedWork W2113187853 @default.
- W2913372637 hasRelatedWork W2352188270 @default.
- W2913372637 hasRelatedWork W2353333671 @default.
- W2913372637 hasRelatedWork W2358921590 @default.
- W2913372637 hasRelatedWork W2376603524 @default.
- W2913372637 hasRelatedWork W2376686917 @default.
- W2913372637 hasRelatedWork W2377744543 @default.
- W2913372637 hasRelatedWork W2913372637 @default.
- W2913372637 hasRelatedWork W4375852831 @default.
- W2913372637 hasVolume "8" @default.
- W2913372637 isParatext "false" @default.
- W2913372637 isRetracted "false" @default.
- W2913372637 magId "2913372637" @default.
- W2913372637 workType "article" @default.