FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2913446789> ?p ?o ?g. }

• W2913446789 abstract "Whey acidic protein (WAP) is a major protein identified in the milk of several mammalian species with cysteine-rich domains known as four-disulfide cores (4-DSC). The organization of the eutherian WAP genes is highly conserved through evolution. It has been proposed that WAP could play an important role in regulating the proliferation of mammary epithelial cells. A bacteriostatic activity was also reported. Conversely to the other mammalian species expressing WAP in their milk, camel WAP contains 4 additional amino acid residues at the beginning of the second 4-DSC domain, introducing a phosphorylation site. The aim of this study was to elucidate the origin of this specificity, which possibly impacts its physiological functions.Using LC-ESI-MS, we identified in Camelus bactrianus from Kazakhstan a phosphorylated whey protein, exhibiting a molecular mass (12,596 Da), 32 Da higher than the original WAP (12,564 Da) and co-eluting with WAP. cDNA sequencing revealed a transition G/A, which modifies an amino acid residue of the mature protein (V12 M), accounting for the mass difference observed between WAP genetic variants. We also report the existence of two splicing variants of camel WAP precursors to mRNA, arising from an alternative usage of the canonical splice site recognized as such in the other mammalian species. However, the major camel WAP isoform results from the usage of an unlikely intron cryptic splice site, extending camel exon 3 upstream by 12-nucleotides encoding the 4 additional amino acid residues (VSSP) in which a potentially phosphorylable Serine residue occurs. Combining protein and cDNA sequences with genome data available (NCBI database), we report another feature of the camel WAP gene which displays a very rare GC-AG type intron. This result was confirmed by sequencing a genomic DNA fragment encompassing exon 3 to exon 4, suggesting for the GC donor site a compensatory effect in terms of consensus at the acceptor exon position.Combining proteomic and molecular biology approaches we report: the characterization of a new genetic variant of camel WAP, the usage of an unlikely intron cryptic splice site, and the occurrence of an extremely rare GC-AG type of intron." @default.
• W2913446789 created "2019-02-21" @default.
• W2913446789 creator A5014365210 @default.
• W2913446789 creator A5018026817 @default.
• W2913446789 creator A5045203888 @default.
• W2913446789 creator A5056467193 @default.
• W2913446789 creator A5069677935 @default.
• W2913446789 creator A5088809377 @default.
• W2913446789 date "2019-01-29" @default.
• W2913446789 modified "2023-10-18" @default.
• W2913446789 title "The main WAP isoform usually found in camel milk arises from the usage of an improbable intron cryptic splice site in the precursor to mRNA in which a GC-AG intron occurs" @default.
• W2913446789 cites W1563643924 @default.
• W2913446789 cites W1580175014 @default.
• W2913446789 cites W1895033516 @default.
• W2913446789 cites W1964419046 @default.
• W2913446789 cites W1971478527 @default.
• W2913446789 cites W1978618605 @default.
• W2913446789 cites W1988272337 @default.
• W2913446789 cites W2001672954 @default.
• W2913446789 cites W2006780044 @default.
• W2913446789 cites W2018289835 @default.
• W2913446789 cites W2020180427 @default.
• W2913446789 cites W2037143951 @default.
• W2913446789 cites W2047905216 @default.
• W2913446789 cites W2053759647 @default.
• W2913446789 cites W2059528306 @default.
• W2913446789 cites W2060325274 @default.
• W2913446789 cites W2062731581 @default.
• W2913446789 cites W2077307041 @default.
• W2913446789 cites W2088151439 @default.
• W2913446789 cites W2090521141 @default.
• W2913446789 cites W2100837269 @default.
• W2913446789 cites W2101249554 @default.
• W2913446789 cites W2108593332 @default.
• W2913446789 cites W2118507136 @default.
• W2913446789 cites W2126733708 @default.
• W2913446789 cites W2136571121 @default.
• W2913446789 cites W2137823730 @default.
• W2913446789 cites W2148343146 @default.
• W2913446789 cites W2149106152 @default.
• W2913446789 cites W2149118168 @default.
• W2913446789 cites W2156620454 @default.
• W2913446789 cites W2168042750 @default.
• W2913446789 cites W2183304400 @default.
• W2913446789 cites W2298750743 @default.
• W2913446789 cites W2801490044 @default.
• W2913446789 doi "https://doi.org/10.1186/s12863-018-0704-x" @default.
• W2913446789 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6350295" @default.
• W2913446789 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30696406" @default.
• W2913446789 hasPublicationYear "2019" @default.
• W2913446789 type Work @default.
• W2913446789 sameAs 2913446789 @default.
• W2913446789 citedByCount "6" @default.
• W2913446789 countsByYear W29134467892020 @default.
• W2913446789 countsByYear W29134467892021 @default.
• W2913446789 countsByYear W29134467892022 @default.
• W2913446789 crossrefType "journal-article" @default.
• W2913446789 hasAuthorship W2913446789A5014365210 @default.
• W2913446789 hasAuthorship W2913446789A5018026817 @default.
• W2913446789 hasAuthorship W2913446789A5045203888 @default.
• W2913446789 hasAuthorship W2913446789A5056467193 @default.
• W2913446789 hasAuthorship W2913446789A5069677935 @default.
• W2913446789 hasAuthorship W2913446789A5088809377 @default.
• W2913446789 hasBestOaLocation W29134467891 @default.
• W2913446789 hasConcept C104317684 @default.
• W2913446789 hasConcept C153911025 @default.
• W2913446789 hasConcept C187882448 @default.
• W2913446789 hasConcept C194583182 @default.
• W2913446789 hasConcept C2776965311 @default.
• W2913446789 hasConcept C2780989783 @default.
• W2913446789 hasConcept C31903555 @default.
• W2913446789 hasConcept C36823959 @default.
• W2913446789 hasConcept C515207424 @default.
• W2913446789 hasConcept C53345823 @default.
• W2913446789 hasConcept C54355233 @default.
• W2913446789 hasConcept C54458228 @default.
• W2913446789 hasConcept C55493867 @default.
• W2913446789 hasConcept C67705224 @default.
• W2913446789 hasConcept C86803240 @default.
• W2913446789 hasConcept C91779695 @default.
• W2913446789 hasConcept C94671646 @default.
• W2913446789 hasConceptScore W2913446789C104317684 @default.
• W2913446789 hasConceptScore W2913446789C153911025 @default.
• W2913446789 hasConceptScore W2913446789C187882448 @default.
• W2913446789 hasConceptScore W2913446789C194583182 @default.
• W2913446789 hasConceptScore W2913446789C2776965311 @default.
• W2913446789 hasConceptScore W2913446789C2780989783 @default.
• W2913446789 hasConceptScore W2913446789C31903555 @default.
• W2913446789 hasConceptScore W2913446789C36823959 @default.
• W2913446789 hasConceptScore W2913446789C515207424 @default.
• W2913446789 hasConceptScore W2913446789C53345823 @default.
• W2913446789 hasConceptScore W2913446789C54355233 @default.
• W2913446789 hasConceptScore W2913446789C54458228 @default.
• W2913446789 hasConceptScore W2913446789C55493867 @default.
• W2913446789 hasConceptScore W2913446789C67705224 @default.
• W2913446789 hasConceptScore W2913446789C86803240 @default.
• W2913446789 hasConceptScore W2913446789C91779695 @default.
• W2913446789 hasConceptScore W2913446789C94671646 @default.
• W2913446789 hasFunder F4320322726 @default.
• W2913446789 hasIssue "1" @default.

• next