Matches in SemOpenAlex for { <https://semopenalex.org/work/W2913666988> ?p ?o ?g. }
- W2913666988 endingPage "856.e5" @default.
- W2913666988 startingPage "845" @default.
- W2913666988 abstract "ADP-ribosylation refers to the addition of one or more ADP-ribose groups onto proteins. The attached ADP-ribose monomers or polymers, commonly known as poly(ADP-ribose) (PAR), modulate the activities of the modified substrates or their binding affinities to other proteins. However, progress in this area is hindered by a lack of tools to investigate this protein modification. Here, we describe a new method named ELTA (enzymatic labeling of terminal ADP-ribose) for labeling free or protein-conjugated ADP-ribose monomers and polymers at their 2'-OH termini using the enzyme OAS1 and dATP. When coupled with various dATP analogs (e.g., radioactive, fluorescent, affinity tags), ELTA can be used to explore PAR biology with techniques routinely used to investigate DNA or RNA function. We demonstrate that ELTA enables the biophysical measurements of protein binding to PAR of a defined length, detection of PAR length from proteins and cells, and enrichment of sub-femtomole amounts of ADP-ribosylated peptides from cell lysates." @default.
- W2913666988 created "2019-02-21" @default.
- W2913666988 creator A5004029993 @default.
- W2913666988 creator A5014448600 @default.
- W2913666988 creator A5024531147 @default.
- W2913666988 creator A5036550290 @default.
- W2913666988 creator A5051765798 @default.
- W2913666988 creator A5060860511 @default.
- W2913666988 creator A5061854810 @default.
- W2913666988 creator A5072507379 @default.
- W2913666988 creator A5076746607 @default.
- W2913666988 creator A5088802895 @default.
- W2913666988 date "2019-02-01" @default.
- W2913666988 modified "2023-10-17" @default.
- W2913666988 title "ELTA: Enzymatic Labeling of Terminal ADP-Ribose" @default.
- W2913666988 cites W1500943849 @default.
- W2913666988 cites W1532086012 @default.
- W2913666988 cites W1537637917 @default.
- W2913666988 cites W1586588960 @default.
- W2913666988 cites W1895248645 @default.
- W2913666988 cites W1950830990 @default.
- W2913666988 cites W1971686407 @default.
- W2913666988 cites W1972350722 @default.
- W2913666988 cites W1975272351 @default.
- W2913666988 cites W1978064890 @default.
- W2913666988 cites W1985660340 @default.
- W2913666988 cites W1990960553 @default.
- W2913666988 cites W1995242155 @default.
- W2913666988 cites W1996995837 @default.
- W2913666988 cites W1998843941 @default.
- W2913666988 cites W2001089067 @default.
- W2913666988 cites W2005458257 @default.
- W2913666988 cites W2007153070 @default.
- W2913666988 cites W2007736465 @default.
- W2913666988 cites W2015842094 @default.
- W2913666988 cites W2018346130 @default.
- W2913666988 cites W2021809952 @default.
- W2913666988 cites W2022350916 @default.
- W2913666988 cites W2026029566 @default.
- W2913666988 cites W2027537529 @default.
- W2913666988 cites W2029765186 @default.
- W2913666988 cites W2033325359 @default.
- W2913666988 cites W2034771321 @default.
- W2913666988 cites W2038131926 @default.
- W2913666988 cites W2044635447 @default.
- W2913666988 cites W2048353021 @default.
- W2913666988 cites W2049216034 @default.
- W2913666988 cites W2049898530 @default.
- W2913666988 cites W2050997074 @default.
- W2913666988 cites W2056133364 @default.
- W2913666988 cites W2056772101 @default.
- W2913666988 cites W2058058143 @default.
- W2913666988 cites W2063258869 @default.
- W2913666988 cites W2065867569 @default.
- W2913666988 cites W2066397124 @default.
- W2913666988 cites W2069226944 @default.
- W2913666988 cites W2086038769 @default.
- W2913666988 cites W2086673973 @default.
- W2913666988 cites W2091118082 @default.
- W2913666988 cites W2094273415 @default.
- W2913666988 cites W2118542406 @default.
- W2913666988 cites W2121571048 @default.
- W2913666988 cites W2127839852 @default.
- W2913666988 cites W2128702837 @default.
- W2913666988 cites W2136708412 @default.
- W2913666988 cites W2144635280 @default.
- W2913666988 cites W2146103350 @default.
- W2913666988 cites W2158389792 @default.
- W2913666988 cites W2159729748 @default.
- W2913666988 cites W2221191766 @default.
- W2913666988 cites W2317027523 @default.
- W2913666988 cites W2332306552 @default.
- W2913666988 cites W2334440167 @default.
- W2913666988 cites W2416703957 @default.
- W2913666988 cites W2427464610 @default.
- W2913666988 cites W2516842853 @default.
- W2913666988 cites W2520780984 @default.
- W2913666988 cites W2525134285 @default.
- W2913666988 cites W2527103315 @default.
- W2913666988 cites W2530540719 @default.
- W2913666988 cites W2559331577 @default.
- W2913666988 cites W2567339323 @default.
- W2913666988 cites W2575865178 @default.
- W2913666988 cites W2584218445 @default.
- W2913666988 cites W2588135595 @default.
- W2913666988 cites W2597062310 @default.
- W2913666988 cites W2604136844 @default.
- W2913666988 cites W2612277616 @default.
- W2913666988 cites W2765249630 @default.
- W2913666988 cites W2768216998 @default.
- W2913666988 cites W2771043670 @default.
- W2913666988 cites W2782599420 @default.
- W2913666988 cites W2789041139 @default.
- W2913666988 cites W2810068379 @default.
- W2913666988 cites W2810241938 @default.
- W2913666988 cites W2889423344 @default.
- W2913666988 cites W4239750803 @default.
- W2913666988 cites W4361741423 @default.