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• W2913709887 startingPage "325" @default.
• W2913709887 abstract "The phloem plays essential roles in the source-to-sink relationship and in long-distance communication, and thereby coordinates growth and development throughout the plant. Here we employed isolation of nuclei tagged in specific cell types coupled with low-input, high-throughput sequencing approaches to analyze the changes of the chromatin modifications H3K4me3 and H3K27me3 and their correlation with gene expression in the phloem companion cells (PCCs) of Arabidopsis(Arabidopsis thaliana) shoots in response to changes in photoperiod. We observed a positive correlation between changes in expression and H3K4me3 levels of genes that are involved in essential PCC functions, including regulation of metabolism, circadian rhythm, development, and epigenetic modifications. By contrast, changes in H3K27me3 signal appeared to contribute little to gene expression changes. These genomic data illustrate the complex gene-regulatory networks that integrate plant developmental and physiological processes in the PCCs. Emphasizing the importance of cell-specific analyses, we identified a previously uncharacterized MORN-motif repeat protein, MORN-MOTIF REPEAT PROTEIN REGULATING FLOWERING1 (MRF1), that was strongly up-regulated in the PCCs in response to inductive photoperiod. The mrf1 mutation delayed flowering, whereas MRF1 overexpression had the opposite effect, indicating that MRF1 acts as a floral promoter." @default.
• W2913709887 created "2019-02-21" @default.
• W2913709887 creator A5042237035 @default.
• W2913709887 creator A5062512325 @default.
• W2913709887 creator A5069325789 @default.
• W2913709887 creator A5076878163 @default.
• W2913709887 creator A5078998093 @default.
• W2913709887 creator A5079366806 @default.
• W2913709887 creator A5080970683 @default.
• W2913709887 date "2019-01-22" @default.
• W2913709887 modified "2023-10-12" @default.
• W2913709887 title "Phloem Companion Cell-Specific Transcriptomic and Epigenomic Analyses Identify MRF1, a Regulator of Flowering" @default.
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